Description

  • Product name

    Recombinant Human RAP1A protein
  • Purity

    >= 85 % SDS-PAGE.
    ab97904 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C
    • Predicted molecular weight

      23 kDa
    • Amino acids

      1 to 181
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab97904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF-TOF
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.077% DTT, 0.316% Tris HCl, 40% Glycerol, 1.16% Sodium chloride

General Info

  • Alternative names

    • C21KG
    • G 22K
    • G-22K
    • GTP binding protein smg p21A
    • GTP-binding protein smg p21A
    • KREV 1
    • KREV1
    • OTTHUMP00000013741
    • RAP 1A
    • RAP1
    • RAP1A
    • RAP1A member of RAS oncogene family
    • RAP1A_HUMAN
    • Ras related protein Krev 1
    • Ras related protein Rap 1A
    • RAS related protein RAP1A
    • Ras-related protein Krev-1
    • Ras-related protein Rap-1A
    • SMGP21
    see all
  • Function

    Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab97904 at approximately 22.8kDa (3µg).

References

ab97904 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

We currently do not carry lysates of cell lines that have been transfected with Rap1. We do however have the recombinant full length mature Human RAP1A (amino acids 1-181). This product contains an N terminal His tag; is 201aa in lenghth with a molecular weight of 22.8kDa. (UniProt ID = P62834).In the absence of the transfected cell line this product would provide an excellent alternative positive control. This product may be found at the following link:

https://www.abcam.com/RAP1A-protein-ab97904.html


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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