Recombinant Human RAP2B protein (ab140538)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
-
Product name
Recombinant Human RAP2B protein -
Purity
> 95 % SDS-PAGE.
ab140538 is purified using conventional chromatography techniques -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMREYKVVVLGSGGVGKSALTVQFVTGS FIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQG FILVYSLVNQQSFQDIKPMRDQIIRVKRYERVPMILVGNKVDLEGEREVS YGEGKALAEEWSCPFMETSAKNKASVDELFAEIVRQMNYAAQPNGDEGCC SACVIL -
Predicted molecular weight
23 kDa including tags -
Amino acids
1 to 183 -
Tags
His tag N-Terminus
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab140538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Mass Spectrometry
-
Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
General Info
-
Alternative names
- MGC20484
- RAP 2B
- RAP2A
see all -
Function
Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells. -
Tissue specificity
Expressed in red blood cells (at protein level). -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Domain
The effector domain mediates the interaction with RUNDC3A. -
Post-translational
modificationsPalmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK. -
Cellular localization
Recycling endosome membrane. Associated with red blood cells-released vesicles. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab140538 has not yet been referenced specifically in any publications.