Description

  • Product name

    Recombinant Human Ras protein
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Tags

      GST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab61239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

General Info

  • Alternative names

    • C BAS/HAS
    • C HA RAS1
    • C-BAS/HAS
    • c-H-ras
    • C-HA-RAS1
    • CTLO
    • GTPase HRas
    • GTPase KRas
    • GTPase NRas
    • H ras
    • H RASIDX
    • H-Ras-1
    • H-RASIDX
    • Ha-Ras
    • HAMSV
    • HRAS
    • HRAS1
    • K ras
    • K RAS2A
    • K RAS2B
    • K RAS4A
    • K RAS4B
    • K-RAS
    • KRAS
    • KRAS1
    • KRAS2
    • N-RAS
    • N-terminally processed
    • NRAS
    • NRAS1
    • p21ras
    • RASH_HUMAN
    • RASH1
    • RASK2
    • Transforming protein p21
    • v Ha ras Harvey rat sarcoma viral oncogene homolog
    • v Ki ras2 Kirsten rat sarcoma viral oncogene homolog
    • v ras neuroblastoma RAS viral oncogene homolog
    see all
  • Function

    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
  • Involvement in disease

    Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
    Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
    Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
    Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
    Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
    Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family.
  • Post-translational
    modifications

    Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
    S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
  • Cellular localization

    Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
  • Information by UniProt

Images

References

ab61239 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Gracias por contactarnos.

La proteína tiene un tag unido a su posición N terminal correspondiente a GST.

Me gustaría informarte de que actualmente tenemos una promoción por la que al enviar cualquier anticuerpo primario os regalamos otro primario Rabbit Monoclonal. Simplemente indica el código RABMAB-XBSMG en el próximo pedido. Para más información picha https://www.abcam.com/index.html?pageconfig=resource&rid=15447.

Espero haberte ayudado, En caso contrario, no dudes en volvernos a contactar.

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Question
Answer

Thank you for your enquiry. There is a N-terminal GST tag on the RAS Protein. If you have any additional questions, please feel free to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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