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  1. Link

    recombinant-human-rbbp4-protein-ab130037.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Recombinant Human RBBP4 protein (ab130037)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human RBBP4 protein (ab130037)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

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    Description

    • Product name

      Recombinant Human RBBP4 protein
      See all RBBP4 proteins and peptides
    • Purity

      > 90 % SDS-PAGE.
      ab130037 is purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      Q09028
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSHMADKEA AFDDAVEERV INEEYKIWKK NTPFLYDLVM THALEWPSLT AQWLPDVTRP EGKDFSIHRL VLGTHTSDEQ NHLVIASVQL PNDDAQFDAS HYDSEKGEFG GFGSVSGKIE IEIKINHEGE VNRARYMPQN PCIIATKTPS SDVLVFDYTK HPSKPDPSGE CNPDLRLRGH QKEGYGLSWN PNLSGHLLSA SDDHTICLWD ISAVPKEGKV VDAKTIFTGH TAVVEDVSWH LLHESLFGSV ADDQKLMIWD TRSNNTSKPS HSVDAHTAEV NCLSFNPYSE FILATGSADK TVALWDLRNL KLKLHSFESH KDEIFQVQWS PHNETILASS GTDRRLNVWD LSKIGEEQSP EDAEDGPPEL LFIHGGHTAK ISDFSWNPNE PWVICSVSED NIMQVWQMAE NIYNDEDPEG SVDPEGQGS
      • Predicted molecular weight

        50 kDa including tags
      • Amino acids

        1 to 425
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-RBBP4 antibody (ab1765)
      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-RBBP4 antibody (ab38135)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-RBBP4 antibody [EPR3411] - ChIP Grade (ab79416)
      • Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

    Specifications

    Our Abpromise guarantee covers the use of ab130037 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.03% DTT, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • CAF I p48
      • CAF-1 subunit C
      • CAF-I 48 kDa subunit
      • CAF-I p48
      • Chromatin assembly factor 1 subunit C
      • Chromatin assembly factor I p48 subunit
      • Chromatin assembly factor/CAF 1 p48 subunit
      • Histone-binding protein RBBP4
      • MSI1 protein homolog
      • Nucleosome-remodeling factor subunit RBAP48
      • NURF55
      • RB binding protein 4 chromatin remodeling factor
      • RbAp 48
      • RBAP48
      • RBBP-4
      • RBBP4
      • RBBP4_HUMAN
      • Retinoblastoma binding protein 4
      • Retinoblastoma binding protein p48
      • Retinoblastoma-binding protein 4
      • Retinoblastoma-binding protein p48
      see all
    • Function

      Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
    • Sequence similarities

      Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
      Contains 6 WD repeats.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q09028 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human RBBP4 protein (ab130037)
      SDS-PAGE - Recombinant Human RBBP4 protein (ab130037)
      15% SDS-PAGE showing ab130037 (3 µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab130037? Please let us know so that we can cite the reference in this datasheet.

    ab130037 has not yet been referenced specifically in any publications.

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