Recombinant Human RBX1 protein (ab157035)

Overview

  • Product name
    Recombinant Human RBX1 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MAAAMDVDTP SGTNSGAGKK RFEVKKWNAV ALWAWDIVVD NCAICRNHIM DLCIECQANQ ASATSEECTV AWGVCNHAFH FHCISRWLKT RQVCPLDNRE WEFQKYGH
    • Molecular weight
      14 kDa including tags
    • Amino acids
      1 to 108

Specifications

Our Abpromise guarantee covers the use of ab157035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 98 % SDS-PAGE.
    Purified by nickel sepharose affinity chromatography.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.02% DTT, 0.6% Tris, 0.29% Sodium chloride

General Info

  • Alternative names
    • BA554C12.1
    • E3 ubiquitin-protein ligase RBX1
    • FLJ60363
    • MGC13357
    • MGC1481
    • OTTHUMP00000028983
    • Protein ZYP
    • Rbx 1
    • Rbx1
    • RBX1_HUMAN
    • Regulator of cullins 1
    • Ring box 1
    • Ring box 1 E3 ubiquitin protein ligase
    • RING box protein 1
    • RING finger protein
    • RING finger protein 75
    • RING-box protein 1
    • Ringbox protein 1
    • RNF 75
    • RNF75
    • ROC 1
    • ROC1
    • ZYP protein
    see all
  • Function
    E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.
  • Tissue specificity
    Widely expressed.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the RING-box family.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE image of ab157035 Lane 1: MW markers (top to bottom) 200, 116, 97, 66, 55, 45, 36, 29, 24, 20, 14.2, 6.5 kDa. Lane 2: ab157035 (2µg)

References

ab157035 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
This recombinant human ROC1 protein (ab157035) is used as a positive control to check its expression in U266 cells
Username

Abcam user community

Verified customer

Submitted Sep 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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