Description

  • Product name

    Recombinant Human RBX1 protein
  • Purity

    > 98 % SDS-PAGE.
    Purified by nickel sepharose affinity chromatography.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MAAAMDVDTP SGTNSGAGKK RFEVKKWNAV ALWAWDIVVD NCAICRNHIM DLCIECQANQ ASATSEECTV AWGVCNHAFH FHCISRWLKT RQVCPLDNRE WEFQKYGH
    • Predicted molecular weight

      14 kDa including tags
    • Amino acids

      1 to 108

Specifications

Our Abpromise guarantee covers the use of ab157035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.02% DTT, 0.6% Tris, 0.29% Sodium chloride

General Info

  • Alternative names

    • BA554C12.1
    • E3 ubiquitin-protein ligase RBX1
    • FLJ60363
    • MGC13357
    • MGC1481
    • OTTHUMP00000028983
    • Protein ZYP
    • Rbx 1
    • Rbx1
    • RBX1_HUMAN
    • Regulator of cullins 1
    • Ring box 1
    • Ring box 1 E3 ubiquitin protein ligase
    • RING box protein 1
    • RING finger protein
    • RING finger protein 75
    • RING-box protein 1
    • Ringbox protein 1
    • RNF 75
    • RNF75
    • ROC 1
    • ROC1
    • ZYP protein
    see all
  • Function

    E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.
  • Tissue specificity

    Widely expressed.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the RING-box family.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE image of ab157035 Lane 1: MW markers (top to bottom) 200, 116, 97, 66, 55, 45, 36, 29, 24, 20, 14.2, 6.5 kDa. Lane 2: ab157035 (2µg)

References

ab157035 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
This recombinant human ROC1 protein (ab157035) is used as a positive control to check its expression in U266 cells

Abcam user community

Verified customer

Submitted Sep 21 2016

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