Recombinant Human RCC1 protein (ab90625)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human RCC1 protein -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab90625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.60
Constituents: 0.29% Sodium chloride, 0.476% HEPES, 0.0308% DTE (1,4-Dithioerythritol)
General Info
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Alternative names
- Cell cycle regulatory protein
- CHC 1
- CHC1
see all -
Function
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly. -
Sequence similarities
Contains 7 RCC1 repeats. -
Post-translational
modificationsN-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran. -
Cellular localization
Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis. - Information by UniProt
Images
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All lanes : Anti-RCC1 antibody (ab70749) at 1/2000 dilution
Lane 1 :Recombinant Human RCC1 protein (ab90625) at 0.01 µg
Lane 2 :Recombinant Human RCC1 protein (ab90625) at 0.1 µg
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution (Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP))
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 8 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab90625 has been referenced in 2 publications.
- Fasci D et al. Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei. Mol Cell Proteomics 17:2018-2033 (2018). PubMed: 30021884
- Binkert M et al. Revisiting chromatin binding of the Arabidopsis UV-B photoreceptor UVR8. BMC Plant Biol 16:42 (2016). PubMed: 26864020