Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human Renin protein
See all Renin proteins and peptides
The specific activity is > 2 U/mg. The specific activity of active Renin was assayed using a Renin Activity Fluorometric Assay Kit
Purity>= 95 % SDS-PAGE.
Expression systemHEK 293 cells
Protein lengthFull length protein
Predicted molecular weight43 kDa including tags
TagsHis tag C-Terminus
Additional sequence information8 x His tag
Our Abpromise guarantee covers the use of ab285713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is manufactured by BioVision, an Abcam company and was previously called 6300 Human CellExp™ Renin, Human Recombinant. 6300-50 is the same size as the 50 µg size of ab285713.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.788% Tris HCl, 0.292% Sodium chloride, 5% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.
- Angiotensin forming enzyme
- Angiotensin forming enzyme precursor
FunctionRenin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Involvement in diseaseDefects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
Sequence similaritiesBelongs to the peptidase A1 family.
Cellular localizationSecreted. Membrane. Associated to membranes via binding to ATP6AP2.
- Information by UniProt
Renin Enzyme activity: The specific activity of activated Renin is > 2 U/mg assayed by using a Renin Activity Fluorometric Assay Kit. Renin concentration was 0.1mg/ml
SDS-PAGE for ab285713 (4-20%) of active and pro Renin: 2 µg of the recombinant Renin and prorenin is loaded under reducing conditions and stained with Coomassie Blue.
Lane M: MW Marker
Lane 1: Active Renin
Lane 2: Prorenin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab285713 has not yet been referenced specifically in any publications.