Recombinant human Renin protein (ab285713)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human Renin protein
See all Renin proteins and peptides -
Biological activity
The specific activity is > 2 U/mg. The specific activity of active Renin was assayed using a Renin Activity Fluorometric Assay Kit
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Purity
>= 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
43 kDa including tags -
Tags
His tag C-Terminus -
Additional sequence information
8 x His tag
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Specifications
Our Abpromise guarantee covers the use of ab285713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 6300 Human CellExp™ Renin, Human Recombinant. 6300-50 is the same size as the 50 µg size of ab285713.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.788% Tris HCl, 0.292% Sodium chloride, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.
General Info
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Alternative names
- Angiotensin forming enzyme
- Angiotensin forming enzyme precursor
- Angiotensinogenase
see all -
Function
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. -
Involvement in disease
Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - Information by UniProt
Images
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Functional Studies - Recombinant human Renin protein (ab285713)
Renin Enzyme activity: The specific activity of activated Renin is > 2 U/mg assayed by using a Renin Activity Fluorometric Assay Kit. Renin concentration was 0.1mg/ml
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SDS-PAGE - Recombinant human Renin protein (ab285713)SDS-PAGE for ab285713 (4-20%) of active and pro Renin: 2 µg of the recombinant Renin and prorenin is loaded under reducing conditions and stained with Coomassie Blue.
Lane M: MW Marker
Lane 1: Active Renin
Lane 2: Prorenin.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab285713 has not yet been referenced specifically in any publications.
