Recombinant Human Renin Receptor protein (ab153053)

Reviews (1) Submit a question
SDS-PAGE - Recombinant Human Renin Receptor protein (ab153053)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: ELISA, WB

    Description

    • Product name

      Recombinant Human Renin Receptor protein
      See all Renin Receptor proteins and peptides

    • Expression system

      Wheat germ

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MAVFVVLLALVAGVLGNEFSILKSPGSVVFRNGNWPIPGERIPDVAALSM GFSVKEDLSWPGLAVGNLFHRPRATVMVMVKGVNKLALPPGSVISYPLEN AVPFSLDSVANSIHSLFSEETPVVLQLAPSEERVYMVGKANSVFEDLSVT LRQLRNRLFQENSVLSSLPLNSLSRNNEVDLLFLSELQVLHDISSLLSRH KHLAKDHSPDLYSLELAGLDEIGKRYGEDSEQFRDASKILVDALQKFADD MYSLYGGNAVVELVTVKSFDTSLIRKTRTILEAKQAKNPASPYNLAYKYN FEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD

      • Amino acids

        1 to 350

      • Tags

        GST tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab153053 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Western blot

    • Form

      Liquid

    • Additional notes

      This product was previously labelled as POU3F2.

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • APT6M8 9
      • APT6M8-9
      • ATP6AP2
      • ATP6IP2
      • ATP6M8-9
      • ATPase H(+)-transporting lysosomal accessory protein 2
      • ATPase H(+)-transporting lysosomal-interacting protein 2
      • ATPase H+ transporting lysosomal accessory protein 2
      • ATPase H+ transporting lysosomal interacting protein 2
      • ATPase H+ transporting lysosomal vacuolar proton pump membrane sector associated protein M8 9
      • ATPase membrane sector associated protein M8 9
      • ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8 9
      • CAPER
      • ELDF10
      • Embryonic liver differentiation factor 10
      • ER localized type I transmembrane adaptor
      • ER-localized type I transmembrane adaptor
      • HT028
      • M8 9
      • M8-9
      • MGC99577
      • MRXE
      • MSTP009
      • N14F
      • Renin receptor
      • Renin/prorenin receptor
      • RENR_HUMAN
      • V ATPase M8 9 subunit
      • V ATPase M8.9 subunit
      • V-ATPase M8.9 subunit
      • Vacuolar ATP synthase membrane sector associated protein M8 9
      • Vacuolar ATP synthase membrane sector-associated protein M8-9
      • vacuolar proton ATP synthase membrane sector associated protein M8 9
      • XMRE
      see all

    • Function

      Functions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS).

    • Tissue specificity

      Expressed in brain, heart, placenta, liver, kidney and pancreas. Barely detectable in lung and skeletal muscles. In the kidney cortex it is restricted to the mesangium of glomeruli. In the coronary and kidney artery it is expressed in the subendothelium, associated to smooth muscles where it colocalizes with REN. Expressed in vascular structures and by syncytiotrophoblast cells in the mature fetal placenta.

    • Involvement in disease

      Defects in ATP6AP2 are a cause of mental retardation X-linked with epilepsy (MRXE) [MIM:300423]. MRXE is a syndromic mental retardation. Patients manifest mild to moderate mental retardation associated with epilepsy, delays in motor milestones and speech acquisition in infancy.

    • Post-translational
      modifications

      Phosphorylated.

    • Cellular localization

      Membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Renin Receptor protein (ab153053)
      SDS-PAGE - Recombinant Human Renin Receptor protein (ab153053)
      ab153053 on a 12.5% SDS-PAGE stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab153053? Please let us know so that we can cite the reference in this datasheet.

    ab153053 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Evaluation of recombinant ATP6IP2 effect on CHO-M2 cell line viability

     Excellent
    Abreviews
    abreview image
    Application
    Other - Do not use
    The effect of recombinant protein on cell viability is assessed using MTS viability determination method on cultured CHO-M2 cells, incubated 42h in presence of different concentrations (45.5 – 22.7 – 11.4 – 5.7 – 2.8 – 1.4 nM) of rATP6IP2.

    CONCLUSION
    The viability is similar for the different concentration of rATP6IP2. It doesn’t affect cell viability.

    Abcam user community

    Verified customer

    Submitted Aug 26 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com