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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Recombinant Human Reptin/TIP49B/RUVB2 protein (His tag) (ab225966)

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SDS-PAGE - Recombinant Human Reptin/TIP49B/RUVB2 protein (His tag) (ab225966)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human Reptin/TIP49B/RUVB2 protein (His tag)
    • Purity

      > 90 % SDS-PAGE.

    • Expression system

      Escherichia coli
    • Accession

      Q9Y230
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        ATVTATTKVPEIRDVTRIERIGAHSHIRGLGLDDALEPRQASQGMVGQLA ARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFT AIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPA TGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGK ISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVIN SRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEV HMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLL DRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRY AIQLITAASLVCRKRKGTEVQVDDIKRVYSLFLDESRSTQYMKEYQDAFL FNELKGETMDTS
      • Predicted molecular weight

        67 kDa including tags
      • Amino acids

        2 to 463
      • Tags

        His tag N-Terminus
      • Additional sequence information

        6xHis-SUMO tag at the N-terminus.

    Associated products

    • Related Products

      • Anti-Reptin/TIP49B/RUVB2 antibody (ab137762)
      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)] (ab196027)
      • Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab225966 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituents: 50% Glycerol (glycerin, glycerine), Tris buffer

    General Info

    • Alternative names

      • 48 kDa TATA box-binding protein-interacting protein
      • 48 kDa TBP-interacting protein
      • 48-kDa TATA box-binding protein-interacting protein
      • 48-kDa TBP-interacting protein
      • 51 kDa erythrocyte cytosolic protein
      • CGI-46
      • EC=3.6.1.-
      • ECP-51
      • ECP51
      • Erythrocyte cytosolic protein, 51-KD
      • INO80 complex subunit J
      • INO80J
      • MGC144733
      • MGC144734
      • MGC52995
      • mp47
      • p47
      • p47 protein
      • Repressing pontin 52
      • REPTIN
      • Reptin 52
      • RuvB (E coli homolog)-like 2
      • RUVB, E. coli, homolog-like 2
      • RuvB-like 2
      • RuvB-like 2 (E. coli)
      • RuvB-like protein 2
      • RUVB2
      • RUVB2_HUMAN
      • RUVBL2
      • RVB2
      • TAP54-beta
      • TATA box-binding protein-interacting protein, 48-KD
      • TBP-interacting protein, 48-KD
      • TIH2
      • TIP48
      • TIP49b
      • TIP60-associated protein 54-beta
      • wu:fi25f01
      • zreptin
      see all
    • Function

      Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.
    • Tissue specificity

      Ubiquitously expressed. Highly expressed in testis and thymus.
    • Sequence similarities

      Belongs to the ruvB family.
    • Domain

      The C-terminal domain is required for association with ATF2.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes.
    • Target information above from: UniProt accession Q9Y230 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Reptin/TIP49B/RUVB2 protein (His tag) (ab225966)
      SDS-PAGE - Recombinant Human Reptin/TIP49B/RUVB2 protein (His tag) (ab225966)

      (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225966 with 5% enrichment gel and 15% separation gel.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab225966? Please let us know so that we can cite the reference in this datasheet.

    ab225966 has not yet been referenced specifically in any publications.

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