Recombinant Human Repulsive Guidance Molecule A protein (His tag) (ab196086)
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Description
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Product name
Recombinant Human Repulsive Guidance Molecule A protein (His tag)
See all Repulsive Guidance Molecule A proteins and peptides -
Purity
> 95 % SDS-PAGE.
The purity of ab196086 is greater than 95%, as determined by SEC-HPLC and reducing SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MNHKVHHHHHHMPHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLP GSAATATSKLTIIFKNFQECVDQKVYQAEMDELPAAFVDGSKNGGDKHGA NSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEVVNAVED WDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFP YETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHS NKDKLHLYER TRDLPG -
Predicted molecular weight
30 kDa including tags -
Amino acids
169 to 422 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab196086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
HPLC
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C.
pH: 7.40
Constituents: 0.02% DTT, 99% PBS
0.2 µM filtered solution
General Info
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Alternative names
- Repulsive guidance molecule A
- RGM
- RGM domain family member A
see all -
Function
Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. -
Sequence similarities
Belongs to the repulsive guidance molecule (RGM) family. -
Cellular localization
Cell membrane. - Information by UniProt
Datasheets and documents
References
ab196086 has not yet been referenced specifically in any publications.
