Overview

  • Product name

    Recombinant Human RFC1 protein
  • Protein length

    Protein fragment

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      27 kDa
    • Amino acids

      775 to 1018
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab127089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride

  • Reconstitution
    Reconstitute with water to desired concentration.

General Info

  • Alternative names

    • A1
    • A1 140 kDa subunit
    • A1 P145 Activator 1 large subunit
    • Activator 1 140 kDa subunit
    • Activator 1 large subunit
    • Activator 1 subunit 1
    • DNA binding Protein PO GA
    • DNA-binding protein PO-GA
    • MHC binding factor beta
    • MHCBFB
    • PO GA
    • RECC1
    • Replication factor C
    • Replication factor C (activator 1) 1, 145kDa
    • Replication factor C 140 kDa subunit
    • Replication factor C large subunit
    • Replication factor C subunit 1
    • Replication factor C1
    • RF-C 140 kDa subunit
    • RFC
    • RFC1
    • RFC1_HUMAN
    • RFC140
    • RFC140 Replication Factor C 140 kDa subunit
    see all
  • Function

    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
    Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
  • Tissue specificity

    Wide tissue distribution. Undetectable in placental tissue.
  • Sequence similarities

    Belongs to the activator 1 large subunit family.
    Contains 1 BRCT domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt

References

ab127089 has not yet been referenced specifically in any publications.

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