Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human Rffl protein (Active)
The specific activity of ab268947 was 18 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.
Purity> 70 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMWATCCNWFCLDGQPEEVPPPQGARMQAYSNPGYSSFPSPTGLEPSCKSC GAHFANTARKQTCLDCKKNFCMTCSSQVGNGPRLCLLCQRFRATAFQREE LMKMKVKDLRDYLSLHDISTEMCREKEELVLLVLGQQPVISQEDRTRAST LSPDFPEQQAFLTQPHSSMVPPTSPNLPSSSAQATSVPPAQVQENQQANG HVSQDQEEPVYLESVARVPAEDETQSIDSEDSFVPGRRASLSDLTDLEDI EGLTVRQLKEILARNFVNYKGCCEKWELMERVTRLYKDQKGLQHLVSGAE DQNGGAVPSGLEENLCKICMDSPIDCVLLECGHMVTCTKCGKRMNECPIC RQYVIRAVHVFRS
Molecular weight information73 kDa by SDS-PAGE
Amino acids1 to 363
TagsGST tag N-Terminus
Additional sequence informationNM_001017368
Our Abpromise guarantee covers the use of ab268947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Caspase regulator CARP2
FunctionHas E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates.
Tissue specificityUbiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 FYVE-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 2 SAP domains.
modificationsRapidly degraded after stimulation with TNFSF10, probably by caspases.
Auto-ubiquitinated (in vitro).
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268947 has not yet been referenced specifically in any publications.