Recombinant Human RFWD3 protein (ab162990)

Overview

  • Product name

    Recombinant Human RFWD3 protein
  • Protein length

    Protein fragment

Description

  • Nature

    Recombinant
  • Source

    Wheat germ
  • Amino Acid Sequence
    • Species

      Human
    • Sequence

      DTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANS ALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHTYKWE
    • Amino acids

      651 to 749
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab162990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • BC027246
    • E3 ubiquitin protein ligase RFWD3
    • E3 ubiquitin-protein ligase RFWD3
    • FLJ10520
    • MGC27888
    • RFWD3
    • RFWD3_HUMAN
    • Ring finger and WD repeat domain 3
    • RING finger and WD repeat domain containing protein 3
    • RING finger and WD repeat domain-containing protein 3
    • RING finger protein 201
    • RNF201
    see all
  • Function

    E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
    Contains 3 WD repeats.
  • Post-translational
    modifications

    Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53.
  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • ab162990 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab162990 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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