The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
Gz selective GTPase activating protein
Gz-selective GTPase-activating protein
Regulator of G protein signaling 20
Regulator of G-protein signaling 20
Regulator of G-protein signaling Z1
Regulator of Gz selective protein signaling 1
Regulator of Gz-selective protein signaling 1
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.
Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.
Contains 1 RGS domain.
Fatty acylated. Heavily palmitoylated in the cysteine string motif. N- and O-glycosylated in synapsomal membranes. Serine phosphorylated in synapsomal membranes. Sumoylated by SUMO1 and SUM02 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits.
Membrane. Nucleus. Cytoplasm. Shuttles between the cytoplasm/cell membrane and the nucleus (By similarity). Anchored to the membrane through palmitoylation.