Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: WB, SDS-PAGE
Product nameRecombinant Human RhoA protein
See all RhoA proteins and peptides
Purity> 85 % SDS-PAGE.
The purity was determined to be >85% by densitometry
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight23 kDa including tags
Amino acids1 to 193
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab101594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 1.02% Imidazole
Constituents: 1.045% MOPS, 0.00174% PMSF, 0.00385% DTT, 25% Glycerol, 1.74% Sodium chloride
- Aplysia ras related homolog 12
FunctionRegulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
DomainThe basic-rich region is essential for yopT recognition and cleavage.
modificationsSubstrate for botulinum ADP-ribosyltransferase.
Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
Cellular localizationCell membrane. Cytoplasm > cytoskeleton.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab101594 has been referenced in 1 publication.
- Welsch ME et al. Multivalent Small-Molecule Pan-RAS Inhibitors. Cell 168:878-889.e29 (2017). PubMed: 28235199