Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human Ribonuclease 3/ECP protein (denatured)
Purity> 90 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSHMRPPQFTRAQWFA IQHISLNPPRCTIAMRAINNYRWRCKNQNTFLRTTFANVVNVCGNQSIRC PHNRTLNNCHRSRFRVPLLHCDLINPGAQNISNCRYADRPGRRFYVVACD NRDPRDSPRYPVVPVHLDTTI
Predicted molecular weight20 kDa including tags
Amino acids28 to 160
TagsHis-DDDDK tag N-Terminus
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
Our Abpromise guarantee covers the use of ab137138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as Ribonuclease 3
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 2.4% Urea, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine)
- Cytotoxic ribonuclease
FunctionCytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity.
Sequence similaritiesBelongs to the pancreatic ribonuclease family.
Cellular localizationCytoplasmic granule. Matrix of eosinophil's large specific granule.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab137138 has not yet been referenced specifically in any publications.