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    recombinant-human-ring1-protein-ab112348.pdf

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Epigenetics and Nuclear Signaling Chromatin Remodeling Polycomb Silencing PRC1
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Recombinant Human RING1 protein (ab112348)

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SDS-PAGE - Recombinant Human RING1 protein (ab112348)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: SDS-PAGE, WB, ELISA

    Description

    • Product name

      Recombinant Human RING1 protein
    • Biological activity

      useful for Antibody Production and Protein Array
    • Expression system

      Wheat germ
    • Accession

      Q06587
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRS GNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLS RLHNQQALSSSIEEGLRMQAMHRAQRVRRPIPGSDQTTTMSGGEGEPGEG EGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVGTGGGGTGGVGGGAGS EDSGDRGGTLGGGTLGPPSPPGAPSPPEPGGEIELVFRPHPLLVEKGEYC QTRYVKTTGNATVDHLSKYLALRIALERRQQQEAGEPGGPGGGASDTGGP DGCGGEGGGAGGGDGPEEPALPSLEGVSEKQYTIYIAPGGGAFTTLNGSL TLELVNEKFWKVSRPLELCYAPTKDPK
      • Predicted molecular weight

        67 kDa including tags
      • Amino acids

        1 to 377
      • Tags

        GST tag N-Terminus

    Associated products

    • Related Products

      • Anti-RING1 antibody (ab32644)

    Specifications

    Our Abpromise guarantee covers the use of ab112348 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

      ELISA

    • Form

      Liquid
    • Additional notes

      useful for Antibody Production and Protein Array
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

      Glutathione is reduced

    General Info

    • Alternative names

      • Ring1A
      • E3 ubiquitin-protein ligase RING1
      • Polycomb complex protein RING1
      • Really interesting new gene 1 protein
      • RING finger protein 1
      • Ring1
      • RING1_HUMAN
      • RING1A
      • Rnf1
      • Transcription repressor Ring1A
      see all
    • Function

      Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Contains 1 RING-type zinc finger.
    • Cellular localization

      Nucleus speckle.
    • Target information above from: UniProt accession Q06587 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human RING1 protein (ab112348)
      SDS-PAGE - Recombinant Human RING1 protein (ab112348)
      12.5% SDS-PAGE showing ab112348 at approximately 67.47kDa stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab112348? Please let us know so that we can cite the reference in this datasheet.

    ab112348 has not yet been referenced specifically in any publications.

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