Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Accession
      Q06587
    • Species
      Human
    • Sequence
      MDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRS GNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLS RLHNQQALSSSIEEGLRMQAMHRAQRVRRPIPGSDQTTTMSGGEGEPGEG EGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVGTGGGGTGGVGGGAGS EDSGDRGGTLGGGTLGPPSPPGAPSPPEPGGEIELVFRPHPLLVEKGEYC QTRYVKTTGNATVDHLSKYLALRIALERRQQQEAGEPGGPGGGASDTGGP DGCGGEGGGAGGGDGPEEPALPSLEGVSEKQYTIYIAPGGGAFTTLNGSL TLELVNEKFWKVSRPLELCYAPTKDPK
    • Molecular weight
      67 kDa including tags
    • Amino acids
      1 to 377
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab112348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    useful for Antibody Production and Protein Array
  • Applications

    SDS-PAGE

    Western blot

    ELISA

  • Form
    Liquid
  • Additional notes
    useful for Antibody Production and Protein Array
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl
    Note: Glutathione is reduced

General Info

  • Alternative names
    • Ring1A
    • E3 ubiquitin-protein ligase RING1
    • Polycomb complex protein RING1
    • Really interesting new gene 1 protein
    • RING finger protein 1
    • Ring1
    • RING1_HUMAN
    • RING1A
    • Rnf1
    • Transcription repressor Ring1A
    see all
  • Function
    Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Nucleus speckle.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human RING1 protein (ab112348)
    SDS-PAGE - Recombinant Human RING1 protein (ab112348)
    12.5% SDS-PAGE showing ab112348 at approximately 67.47kDa stained with Coomassie Blue.

References

ab112348 has not yet been referenced specifically in any publications.

Publishing research using ab112348? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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