Recombinant human RIP2 protein (ab60879)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human RIP2 protein
See all RIP2 proteins and peptides -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Amino acids
1 to 299
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CARD 3
- CARD carrying kinase
- CARD containing ICE associated kinase
see all -
Function
Activates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B. -
Tissue specificity
Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 CARD domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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Functional Studies - Recombinant human RIP2 protein (ab60879)The specific activity of RIP2 (ab60879) was determined to be 20 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human RIP2 protein (ab60879)SDS PAGE analysis of ab60879 -
SDS-PAGE - Recombinant human RIP2 protein (ab60879)SDS PAGE analysis of ab60879 -
Functional Studies - Recombinant human RIP2 protein (ab60879)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human RIP2 protein (ab60879)ab60879 on SDS-PAGE, MW ~59 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab60879 has not yet been referenced specifically in any publications.
