Recombinant human RIP2 protein (ab60879)

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Functional Studies - Recombinant human RIP2 protein (ab60879)
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)
  • Functional Studies - Recombinant human RIP2 protein (ab60879)
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% Densitometry
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human RIP2 protein
    See all RIP2 proteins and peptides

  • Purity

    > 90 % Densitometry.
    Affinity purified.

  • Expression system

    Baculovirus infected Sf9 cells

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Amino acids

      1 to 299

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid

  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CARD 3
    • CARD carrying kinase
    • CARD containing ICE associated kinase
    • CARD containing IL 1 beta ICE kinase
    • CARD containing IL1 beta ICE kinase
    • CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase
    • CARD containing interleukin 1 beta converting enzyme associated kinase
    • CARD-containing IL-1 beta ICE-kinase
    • CARD-containing interleukin-1 beta-converting enzyme-associated kinase
    • CARD3
    • CARDIAK
    • CCK
    • CLARP kinase
    • GIG 30
    • GIG30
    • Growth inhibiting gene 30
    • Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase
    • Receptor interacting protein 2
    • Receptor interacting serine threonine kinase 2
    • Receptor interacting serine/threonine protein kinase 2
    • Receptor-interacting protein 2
    • Receptor-interacting serine/threonine-protein kinase 2
    • RICK
    • RIP 2
    • RIP like interacting CLARP kinase
    • RIP-2
    • RIP-like-interacting CLARP kinase
    • RIPK 2
    • Ripk2
    • RIPK2_HUMAN
    • TNFRSF
    • Tyrosine-protein kinase RIPK2
    • UNQ277/PRO314/PRO34092
    see all

  • Function

    Activates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B.

  • Tissue specificity

    Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.

  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 CARD domain.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human RIP2 protein (ab60879)
    Functional Studies - Recombinant human RIP2 protein (ab60879)
    The specific activity of RIP2 (ab60879) was determined to be 20 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    SDS PAGE analysis of ab60879
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    SDS PAGE analysis of ab60879
  • Functional Studies - Recombinant human RIP2 protein (ab60879)
    Functional Studies - Recombinant human RIP2 protein (ab60879)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    ab60879 on SDS-PAGE, MW ~59 kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab60879? Please let us know so that we can cite the reference in this datasheet.

ab60879 has not yet been referenced specifically in any publications.

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