For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    recombinant-human-rip2-protein-ab60879.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Intracellular Kinases
Share by email

Recombinant human RIP2 protein (ab60879)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Functional Studies - Recombinant human RIP2 protein (ab60879)
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)
  • Functional Studies - Recombinant human RIP2 protein (ab60879)
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human RIP2 protein
    See all RIP2 proteins and peptides
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      1 to 299

Associated products

  • Substrate reagent

    • Native Pig Myelin Basic Protein (ab64311)

Specifications

Our Abpromise guarantee covers the use of ab60879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CARD 3
    • CARD carrying kinase
    • CARD containing ICE associated kinase
    • CARD containing IL 1 beta ICE kinase
    • CARD containing IL1 beta ICE kinase
    • CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase
    • CARD containing interleukin 1 beta converting enzyme associated kinase
    • CARD-containing IL-1 beta ICE-kinase
    • CARD-containing interleukin-1 beta-converting enzyme-associated kinase
    • CARD3
    • CARDIAK
    • CCK
    • CLARP kinase
    • GIG 30
    • GIG30
    • Growth inhibiting gene 30
    • Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase
    • Receptor interacting protein 2
    • Receptor interacting serine threonine kinase 2
    • Receptor interacting serine/threonine protein kinase 2
    • Receptor-interacting protein 2
    • Receptor-interacting serine/threonine-protein kinase 2
    • RICK
    • RIP 2
    • RIP like interacting CLARP kinase
    • RIP-2
    • RIP-like-interacting CLARP kinase
    • RIPK 2
    • Ripk2
    • RIPK2_HUMAN
    • TNFRSF
    • Tyrosine-protein kinase RIPK2
    • UNQ277/PRO314/PRO34092
    see all
  • Function

    Activates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B.
  • Tissue specificity

    Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 CARD domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession O43353 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human RIP2 protein (ab60879)
    Functional Studies - Recombinant human RIP2 protein (ab60879)
    The specific activity of RIP2 (ab60879) was determined to be 20 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    SDS PAGE analysis of ab60879
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    SDS PAGE analysis of ab60879
  • Functional Studies - Recombinant human RIP2 protein (ab60879)
    Functional Studies - Recombinant human RIP2 protein (ab60879)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    SDS-PAGE - Recombinant human RIP2 protein (ab60879)
    ab60879 on SDS-PAGE, MW ~59 kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab60879? Please let us know so that we can cite the reference in this datasheet.

    ab60879 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab60879.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.