Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human RIP2 protein (Active)
See all RIP2 proteins and peptides
Biological activitySpecific Activity: ≥15 pmol/min/μg.Assay Conditions: Assay was done in a Kinase buffer using MBP (0.2 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 minutes. The amount of ATP transferred was calculated using Kinase-Glo reagent.
Purity>= 90 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceNGEAICSALPTIPYHKLADLRYLSRGASGTVSSARHADWRVQVAVKHLHI HTPLLDSERKDVLREAEILHKARFSYILPILGICNEPEFLGIVTEYMPNG SLNELLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTPPLLHHDLKTQN ILLDNEFHVKIADFGLSKWRMMSLSQSRSSKSAPEGGTIIYMPPENYEPG QKSRASIKHDIYSYAVITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVIN EESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEE
Predicted molecular weight61 kDa including tags
Amino acids2 to 299
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab271734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.63% Tris HCl
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- CARD 3
- CARD carrying kinase
- CARD containing ICE associated kinase
FunctionActivates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B.
Tissue specificityDetected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 CARD domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271734 has not yet been referenced specifically in any publications.