Recombinant human RIP2 protein (Active) (ab271734)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
-
Product name
Recombinant human RIP2 protein (Active)
See all RIP2 proteins and peptides -
Biological activity
Specific Activity: ≥15 pmol/min/μg.Assay Conditions: Assay was done in a Kinase buffer using MBP (0.2 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 minutes. The amount of ATP transferred was calculated using Kinase-Glo reagent. -
Purity
>= 90 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
NGEAICSALPTIPYHKLADLRYLSRGASGTVSSARHADWRVQVAVKHLHI HTPLLDSERKDVLREAEILHKARFSYILPILGICNEPEFLGIVTEYMPNG SLNELLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTPPLLHHDLKTQN ILLDNEFHVKIADFGLSKWRMMSLSQSRSSKSAPEGGTIIYMPPENYEPG QKSRASIKHDIYSYAVITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVIN EESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEE -
Predicted molecular weight
61 kDa including tags -
Amino acids
2 to 299 -
Tags
GST tag N-Terminus
-
Specifications
Our Abpromise guarantee covers the use of ab271734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
-
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.63% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- CARD 3
- CARD carrying kinase
- CARD containing ICE associated kinase
see all -
Function
Activates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B. -
Tissue specificity
Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 CARD domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab271734 has not yet been referenced specifically in any publications.