Recombinant Human RNA Helicase A protein (ab114300)

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Description

  • Product name

    Recombinant Human RNA Helicase A protein
    See all RNA Helicase A proteins and peptides

  • Expression system

    Wheat germ

  • Accession

    Q08211

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGDVKNFLYAWCGKRKMTPTYEIRAVGNKNRQKFMCEVQVEGYNYTGMGN STNKKDAQSNAARDFVNYLVRINEIKSEEVPAFGVASPPP

    • Predicted molecular weight

      36 kDa including tags

    • Amino acids

      1 to 90

Specifications

Our Abpromise guarantee covers the use of ab114300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    This protein is best used within three months from the date of receipt.

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • ATP dependent RNA helicase A
    • ATP-dependent RNA helicase A
    • DDX 9
    • DDX9
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9
    • DEAD/H box 9
    • DEAD/H box polypeptide 9
    • DEAH (Asp Glu Ala His) box polypeptide 9
    • DEAH box polypeptide 9
    • DEAH box protein 9
    • DHX 9
    • dhx9
    • DHX9_HUMAN
    • Leukophysin
    • LKP
    • NDH 2
    • NDH II
    • NDH2
    • NDHII
    • Nuclear DNA helicase II
    • RHA
    see all

  • Function

    Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.

  • Sequence similarities

    Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • Domain

    The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.

  • Post-translational
    modifications

    Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
    May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human RNA Helicase A protein (ab114300)
    SDS-PAGE - Recombinant Human RNA Helicase A protein (ab114300)
    12.5% SDS-PAGE image showing ab114300 Stained with Coomassie Blue.

References

ab114300 has not yet been referenced specifically in any publications.

Publishing research using ab114300? Please let us know so that we can cite the reference in this datasheet.

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