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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription Polymerase
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Recombinant Human RNA polymerase II RPB4 protein (denatured) (ab168087)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human RNA polymerase II RPB4 protein (denatured) (ab168087)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human RNA polymerase II RPB4 protein (denatured)
    • Purity

      > 90 % SDS-PAGE.

    • Expression system

      Escherichia coli
    • Accession

      O15514
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSMAAGGSD PRAGDVEEDA SQLIFPKEFE TAETLLNSEV HMLLEHRKQQ NESAEDEQEL SEVFMKTLNY TARFSRFKNR ETIASVRSLL LQKKLHKFEL ACLANLCPET AEESKALIPS LEGRFEDEEL QQILDDIQTK RSFQY
      • Predicted molecular weight

        19 kDa including tags
      • Amino acids

        1 to 142
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab168087 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 2.4% Urea, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • DNA directed RNA polymerase II 16 kDa polypeptide
      • DNA directed RNA polymerase II subunit D
      • DNA directed RNA polymerase II subunit RPB4
      • DNA-directed RNA polymerase II 16 kDa polypeptide
      • DNA-directed RNA polymerase II subunit D
      • DNA-directed RNA polymerase II subunit rpb4
      • HSRBP4
      • HSRPB4
      • polr2d
      • polymerase RNA II DNA directed polypeptide D
      • RBP4
      • RNA polymerase II 16 kDa subunit
      • RNA polymerase II subunit B4
      • RNA polymerase II subunit D
      • RNA polymerase II subunit hsRBP4
      • RPB16
      • RPB4_HUMAN
      see all
    • Function

      DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA.
    • Sequence similarities

      Belongs to the eukaryotic RPB4 RNA polymerase subunit family.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession O15514 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human RNA polymerase II RPB4 protein (denatured) (ab168087)
      SDS-PAGE - Recombinant Human RNA polymerase II RPB4 protein (denatured) (ab168087)
      15% SDS-PAGE analysis of ab168087 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab168087? Please let us know so that we can cite the reference in this datasheet.

    ab168087 has not yet been referenced specifically in any publications.

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