The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
>90% by SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00 Constituents: 2.4% Urea, 0.32% Tris HCl, 10% Glycerol
DNA directed RNA polymerase II 16 kDa polypeptide
DNA directed RNA polymerase II subunit D
DNA directed RNA polymerase II subunit RPB4
DNA-directed RNA polymerase II 16 kDa polypeptide
DNA-directed RNA polymerase II subunit D
DNA-directed RNA polymerase II subunit rpb4
polymerase RNA II DNA directed polypeptide D
RNA polymerase II 16 kDa subunit
RNA polymerase II subunit B4
RNA polymerase II subunit D
RNA polymerase II subunit hsRBP4
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA.
Belongs to the eukaryotic RPB4 RNA polymerase subunit family.