Recombinant human RNF20 protein (ab198645)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 50% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human RNF20 protein -
Biological activity
Assay Conditions: 50 µl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin and ab198645 in 50 mM Tris (pH 7.4) incubate for 30 min at 37°C. Then add to streptavidin coated plates and incubate for 30 min at 37°C. Add antibody against RNF20, incubate 1 hour. Finally, incubate 1 hour with the HRP-labeled secondary antibody.
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Purity
>= 50 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETIKLGGVSSTEELDIRTL QTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFD ENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEG QEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVEL LSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFS KLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNS KGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRLCELEKLRQ DFEEVTTQNEKLKVELRSAVEQVVKETPEYRCMQSQFSVLYNESLQLKAH LDEARTLLHGTRGTHQHQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKE YEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKL REAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKPDSEDLSSQSSASK ASQEDANEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESE KERDSAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYR SAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDAL RKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQ QLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQV VRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQL ELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDN VPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYD TRQRKCPKCNAAFGANDFHRIYIG -
Predicted molecular weight
115 kDa including tags -
Amino acids
2 to 975 -
Tags
His-DDDDK tag N-Terminus -
Additional sequence information
NM_019592.
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Associated products
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Related Products
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-RNF20 antibody [EPR13563(B)] (ab181032)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
Specifications
Our Abpromise guarantee covers the use of ab198645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Contains 80 ng/µl DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- BRE1
- BRE1 A
- BRE1 E3 ubiquitin ligase homolog
see all -
Function
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. -
Tissue specificity
Expressed in the normal brain and also in malignant gliomas (at protein level). -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the BRE1 family.
Contains 1 RING-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab198645 has not yet been referenced specifically in any publications.