Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 50% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human RNF20 protein
Assay Conditions: 50 µl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin and ab198645 in 50 mM Tris (pH 7.4) incubate for 30 min at 37°C. Then add to streptavidin coated plates and incubate for 30 min at 37°C. Add antibody against RNF20, incubate 1 hour. Finally, incubate 1 hour with the HRP-labeled secondary antibody.
Purity>= 50 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceSGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETIKLGGVSSTEELDIRTL QTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFD ENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEG QEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVEL LSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFS KLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNS KGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRLCELEKLRQ DFEEVTTQNEKLKVELRSAVEQVVKETPEYRCMQSQFSVLYNESLQLKAH LDEARTLLHGTRGTHQHQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKE YEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKL REAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKPDSEDLSSQSSASK ASQEDANEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESE KERDSAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYR SAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDAL RKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQ QLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQV VRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQL ELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDN VPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYD TRQRKCPKCNAAFGANDFHRIYIG
Predicted molecular weight115 kDa including tags
Amino acids2 to 975
TagsHis-DDDDK tag N-Terminus
Additional sequence informationNM_019592.
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-RNF20 antibody [EPR13563(B)] (ab181032)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
Our Abpromise guarantee covers the use of ab198645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Contains 80 ng/µl DDDDK peptide.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- BRE1 A
- BRE1 E3 ubiquitin ligase homolog
FunctionComponent of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Tissue specificityExpressed in the normal brain and also in malignant gliomas (at protein level).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the BRE1 family.
Contains 1 RING-type zinc finger.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198645 has not yet been referenced specifically in any publications.