Product nameRecombinant human RNF20 protein
See all RNF20 proteins and peptides
Protein lengthFull length protein
SourceBaculovirus infected Sf9 cells
Amino Acid Sequence
SequenceSGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETIKLGGVSSTEELDIRTL QTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFD ENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEG QEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVEL LSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFS KLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNS KGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRLCELEKLRQ DFEEVTTQNEKLKVELRSAVEQVVKETPEYRCMQSQFSVLYNESLQLKAH LDEARTLLHGTRGTHQHQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKE YEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKL REAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKPDSEDLSSQSSASK ASQEDANEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESE KERDSAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYR SAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDAL RKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQ QLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQV VRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQL ELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDN VPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYD TRQRKCPKCNAAFGANDFHRIYIG
Molecular weight115 kDa including tags
Amino acids2 to 975
TagsHis-DDDDK tag N-Terminus
Additional sequence informationNM_019592.
- Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab1162)
- Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab1257)
- Anti-RNF20 antibody [EPR13563(B)] (ab181032)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag antibody [F-tag-01] - (Equivalent to FLAG antibodies from Sigma) (ab18230)
- Anti-RNF20 antibody (ab33500)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
Our Abpromise guarantee covers the use of ab198645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Assay Conditions: 50 µl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin and ab198645 in 50 mM Tris (pH 7.4) incubate for 30 min at 37°C. Then add to streptavidin coated plates and incubate for 30 min at 37°C. Add antibody against RNF20, incubate 1 hour. Finally, incubate 1 hour with the HRP-labeled secondary antibody.
Purity>= 50 % SDS-PAGE.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol, 0.05% DTT
Contains 80 ng/µl DDDDK peptide.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- BRE1 A
- BRE1 E3 ubiquitin ligase homolog
FunctionComponent of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Tissue specificityExpressed in the normal brain and also in malignant gliomas (at protein level).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the BRE1 family.
Contains 1 RING-type zinc finger.
- Information by UniProt
ab198645 has not yet been referenced specifically in any publications.