Overview

  • Product name

    Recombinant Human RNF4 protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMSTRKRR GGAINSRQAQ KRTREATSTP EISLEAEPIE LVETAGDEIV DLTCESLEPV VVDLTHNDSV VIVDERRRPR RNARRLPQDH ADSCVVSSDD EELSRDRDVY VTTHTPRNAR DEGATGLRPS GTVSCPICMD GYSEIVQNGR LIVSTECGHV FCSQCLRDSL KNANTCPTCR KKINHKRYHP IYI
    • Molecular weight

      24 kDa including tags
    • Amino acids

      1 to 190
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab134611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF
  • Purity

    > 90 % SDS-PAGE.
    ab134611 was purified using conventional chromatography techniques.
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.50
    Constituents: 0.02% DTT, 0.32% Tris HCl, 30% Glycerol, 1.17% Sodium chloride

General Info

  • Alternative names

    • E3 ubiquitin ligase RNF4
    • E3 ubiquitin protein ligase RNF4
    • Protein SNURF
    • RES4 26
    • RING finger protein 4
    • Rnf4
    • RNF4_HUMAN
    • SLX5
    • Small nuclear ring finger protein
    • SNURF
    see all
  • Function

    E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation.
  • Tissue specificity

    Widely expressed at low levels in many tissues; highly expressed in testis.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger domain is required for the ubiquitination of polysumoylated substrates.
  • Post-translational
    modifications

    Sumoylated; conjugated by one or two SUMO1 moieties.
    Autoubiquitinated.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus > PML body. Nucleus > nucleoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab134611 (3µg).

References

ab134611 has not yet been referenced specifically in any publications.

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