Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human Robo4 protein
See all Robo4 proteins and peptides
Purity> 95 % SDS-PAGE.
ab174019 was lyophilized from 0.22 µm filtered solution.
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceQDSPPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQPLSMVPP DPHHLLPDGTLLLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRG ARLSVAVLREDFQIQPRDMVAVVGEQFTLECGPPWGHPEPTVSWWKDGKP LALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRESRAARVSIQE PQDYTEPVELLAVRIQLENVTLLNPDPAEGPKP RPAVWLSWKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAEL GGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEKVPSAPPQEVTLKPGN GTVFVSWVPPPAENHNGIIR GYQVWSLGNT SLPPANWTVV GEQTQLEIAT HMPGSYCVQV AAVTGAGAGE PSRPVCLLLE QAMERATQEP SEHGPWTLEQ LRATLKRPE
Predicted molecular weight49 kDa including tags
Amino acids28 to 469
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab174019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 95% PBS, 5% Trehalose
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is approximately 3-5%.
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
- Magic roundabout
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab174019 has not yet been referenced specifically in any publications.