• Nature
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
    • Amino acids
      17 to 535

Associated products


Our Abpromise guarantee covers the use of ab51415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Activity is lot-specific.

  • Applications

    Functional Studies


  • Form
  • Additional notes

    ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.87% Sodium chloride, 25% Glycerol, 0.00292% EDTA, 0.79% Tris HCl, 0.00385% DTT, 0.00174% PMSF, 0.0038% EGTA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • coiled-coil-containing protein kinase 1
    • coiled-coil-containing protein kinase I
    • MGC131603
    • MGC43611
    • p160 Rhoassociated coiled coil-forming protein kinase
    • p160 ROCK-1
    • p160 ROCK1
    • p160ROCK
    • PRO0435
    • Renal carcinoma antigen NY REN 35
    • Renal carcinoma antigen NY-REN-35
    • Rho associated coiled coil containing protein kinase 1
    • Rho associated protein kinase 1
    • Rho kinase
    • Rho-alpha kinase
    • Rho-associated
    • Rho-associated protein kinase 1
    • ROCK I
    • ROCK-I
    • ROCK1
    • Rok
    • rokalpha
    see all
  • Function
    Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
  • Tissue specificity
    Detected in blood platelets.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 REM (Hr1) repeat.
  • Domain
    The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.
  • Post-translational
    Autophosphorylated on serine and threonine residues.
    Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
  • Cellular localization
    Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.
  • Information by UniProt


  • Kinase activity assay - specific activity 51 nmol/min/mg.
  • Recombinant human ROCK1 protein (tagged)
    Molecular weight 85 kDa


ab51415 has not yet been referenced specifically in any publications.

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