Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human ROCK1 protein
See all ROCK1 proteins and peptides
Activity is lot-specific.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Amino acids17 to 535
Our Abpromise guarantee covers the use of ab51415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.87% Sodium chloride, 25% Glycerol (glycerin, glycerine), 0.00292% EDTA, 0.79% Tris HCl, 0.00385% DTT, 0.00174% PMSF, 0.0038% EGTA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- coiled-coil-containing protein kinase 1
- coiled-coil-containing protein kinase I
FunctionProtein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
Tissue specificityDetected in blood platelets.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.
DomainThe C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.
modificationsAutophosphorylated on serine and threonine residues.
Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
Cellular localizationCytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.
- Information by UniProt
The specific activity of ROCK1 (ab51415) was determined to be 65 nmol/min/mg as per activity assay protocol and was equivalent to 49 nmol/min/mg as per radiometric assay
SDS PAGE analysis of ab51415
Kinase activity assay - specific activity 51 nmol/min/mg.
Recombinant human ROCK1 protein (tagged)
Molecular weight 85 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51415 has not yet been referenced specifically in any publications.