Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% Densitometry
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant human ROCK2 protein
See all ROCK2 proteins and peptides
Biological activityThe specific activity of ab125828 was determined to be 605 nmol/min/mg.
Purity> 95 % Densitometry.
Purity was determined to be >95% by densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Predicted molecular weight66 kDa including tags
Amino acids5 to 554
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab125828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 1.02% Imidazole
Constituents: 0.002% PMSF, 0.71% Sodium phosphate, 0.004% DTT, 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- coiled-coil-containing protein kinase 2
- p164 ROCK 2
FunctionRegulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125828 has not yet been referenced specifically in any publications.