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    recombinant-human-rock2-protein-ab125828.pdf

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Cell Biology Apoptosis Intracellular Kinases
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Recombinant human ROCK2 protein (ab125828)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant human ROCK2 protein (ab125828)
  • Functional Studies - Recombinant human ROCK2 protein (ab125828)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 95% Densitometry
  • Active: Yes
  • Tags: His tag N-Terminus
  • Suitable for: WB, Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human ROCK2 protein
    See all ROCK2 proteins and peptides
  • Biological activity

    The specific activity of ab125828 was determined to be 605 nmol/min/mg.
  • Purity

    > 95 % Densitometry.
    Purity was determined to be >95% by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    O75116
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      66 kDa including tags
    • Amino acids

      5 to 554
    • Tags

      His tag N-Terminus

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
  • Substrate reagent

    • RPS6 peptide (ab204880)

Specifications

Our Abpromise guarantee covers the use of ab125828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.002% PMSF, 0.71% Sodium phosphate, 0.004% DTT, 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • coiled-coil-containing protein kinase 2
    • KIAA0619
    • p164 ROCK 2
    • p164 ROCK-2
    • Rho associated coiled coil containing protein kinase 2
    • Rho associated protein kinase 2
    • Rho associated, coiled coil containing protein kinase II
    • Rho kinase 2
    • Rho-associated
    • Rho-associated protein kinase 2
    • ROCK 2
    • Rock II
    • Rock2
    • ROCK2_HUMAN
    • Rock2m
    • ROK alpha
    • ROKalpha
    see all
  • Function

    Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 REM (Hr1) repeat.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
  • Target information above from: UniProt accession O75116 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant human ROCK2 protein (ab125828)
    SDS-PAGE - Recombinant human ROCK2 protein (ab125828)
    SDS-PAGE analysis of ab125828.
  • Functional Studies - Recombinant human ROCK2 protein (ab125828)
    Functional Studies - Recombinant human ROCK2 protein (ab125828)
    The specific activity of ab125828 was determined to be 605 nmol/min/mg.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab125828? Please let us know so that we can cite the reference in this datasheet.

    ab125828 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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