Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
      O75116
    • Species
      Human
    • Molecular weight
      66 kDa including tags
    • Amino acids
      5 to 554
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125828 was determined to be 605 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 95 % Densitometry.
    Purity was determined to be >95% by densitometry.
  • Form
    Liquid
  • Additional notes

    ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.002% PMSF, 0.71% Sodium phosphate, 0.004% DTT, 25% Glycerol, 1.75% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • coiled-coil-containing protein kinase 2
    • KIAA0619
    • p164 ROCK 2
    • p164 ROCK-2
    • Rho associated coiled coil containing protein kinase 2
    • Rho associated protein kinase 2
    • Rho associated, coiled coil containing protein kinase II
    • Rho kinase 2
    • Rho-associated
    • Rho-associated protein kinase 2
    • ROCK 2
    • Rock II
    • Rock2
    • ROCK2_HUMAN
    • Rock2m
    • ROK alpha
    • ROKalpha
    see all
  • Function
    Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 REM (Hr1) repeat.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human ROCK2 protein (ab125828)
    SDS-PAGE - Recombinant human ROCK2 protein (ab125828)
    SDS-PAGE analysis of ab125828.
  • Functional Studies - Recombinant human ROCK2 protein (ab125828)
    Functional Studies - Recombinant human ROCK2 protein (ab125828)
    The specific activity of ab125828 was determined to be 605 nmol/min/mg.

References

ab125828 has not yet been referenced specifically in any publications.

Publishing research using ab125828? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

There are currently no Customer reviews or Questions for ab125828.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up