Recombinant human ROCK2 protein (ab125828)
- Datasheet
- References
- Protocols
Overview
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Product nameRecombinant human ROCK2 protein
See all ROCK2 proteins and peptides -
Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceBaculovirus infected Sf9 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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Molecular weight66 kDa including tags
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Amino acids5 to 554
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TagsHis tag N-Terminus
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab125828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activityThe specific activity of ab125828 was determined to be 605 nmol/min/mg.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Purity> 95 % Densitometry.
Purity was determined to be >95% by densitometry. -
FormLiquid
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Additional notes
ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.002% PMSF, 0.71% Sodium phosphate, 0.004% DTT, 25% Glycerol, 1.75% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- coiled-coil-containing protein kinase 2
- KIAA0619
- p164 ROCK 2
see all -
FunctionRegulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
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Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationCytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
- Information by UniProt
Images
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SDS-PAGE - Recombinant human ROCK2 protein (ab125828)SDS-PAGE analysis of ab125828. -
Functional Studies - Recombinant human ROCK2 protein (ab125828)The specific activity of ab125828 was determined to be 605 nmol/min/mg.
References
ab125828 has not yet been referenced specifically in any publications.
