Recombinant Human RPB3 protein (ab181910)

Submit a review Submit a question
SDS-PAGE - Recombinant Human RPB3 protein (ab181910)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human RPB3 protein

    • Purity

      > 90 % SDS-PAGE.
      ab181910 was purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      P19387

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMGSMPYANQPTVRITELTDENVKFIIENTD LAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDD IVDKLQYSRDCTCEEFCPECSVEFTLDVRCEDQTRHVTSRDLISNSRVIP VTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPT AGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERF YYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN

      • Predicted molecular weight

        34 kDa including tags

      • Amino acids

        1 to 275

      • Tags

        His tag N-Terminus

      • Additional sequence information

        NP_116558

    Specifications

    Our Abpromise guarantee covers the use of ab181910 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid

    • Additional notes

      Previously labelled as POLR2C.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.02% DTT, 0.58% Sodium chloride

    General Info

    • Alternative names

      • DNA directed RNA polymerase II 33 kDa polypeptide
      • DNA directed RNA polymerase II subunit C
      • DNA directed RNA polymerase II subunit RPB3
      • DNA-directed RNA polymerase II 33 kDa polypeptide
      • DNA-directed RNA polymerase II subunit C
      • DNA-directed RNA polymerase II subunit RPB3
      • hRPB33
      • hsRPB3
      • POLR 2C
      • POLR2 C
      • Polr2c
      • Polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
      • polymerase RNA II DNA directed polypeptide C 33kDa
      • RNA polymerase II polypeptide C
      • RNA polymerase II subunit 3
      • RNA polymerase II subunit B3
      • RPB 3
      • RPB3
      • RPB3_HUMAN
      • RPB31
      • RPB33
      see all

    • Function

      DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft.

    • Sequence similarities

      Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human RPB3 protein (ab181910)
      SDS-PAGE - Recombinant Human RPB3 protein (ab181910)

      15% SDS-PAGE analysis of ab181910 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab181910? Please let us know so that we can cite the reference in this datasheet.

    ab181910 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab181910.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com