Recombinant Human RPB3 protein (ab181910)

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Description

  • Product name

    Recombinant Human RPB3 protein

  • Purity

    > 90 % SDS-PAGE.
    ab181910 was purified using conventional chromatography techniques.

  • Expression system

    Escherichia coli

  • Accession

    P19387

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMGSMPYANQPTVRITELTDENVKFIIENTD LAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDD IVDKLQYSRDCTCEEFCPECSVEFTLDVRCEDQTRHVTSRDLISNSRVIP VTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPT AGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERF YYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN

    • Predicted molecular weight

      34 kDa including tags

    • Amino acids

      1 to 275

    • Tags

      His tag N-Terminus

    • Additional sequence information

      NP_116558

Specifications

Our Abpromise guarantee covers the use of ab181910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid

  • Additional notes

    Previously labelled as POLR2C.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.02% DTT, 0.58% Sodium chloride

General Info

  • Alternative names

    • DNA directed RNA polymerase II 33 kDa polypeptide
    • DNA directed RNA polymerase II subunit C
    • DNA directed RNA polymerase II subunit RPB3
    • DNA-directed RNA polymerase II 33 kDa polypeptide
    • DNA-directed RNA polymerase II subunit C
    • DNA-directed RNA polymerase II subunit RPB3
    • hRPB33
    • hsRPB3
    • POLR 2C
    • POLR2 C
    • Polr2c
    • Polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
    • polymerase RNA II DNA directed polypeptide C 33kDa
    • RNA polymerase II polypeptide C
    • RNA polymerase II subunit 3
    • RNA polymerase II subunit B3
    • RPB 3
    • RPB3
    • RPB3_HUMAN
    • RPB31
    • RPB33
    see all

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft.

  • Sequence similarities

    Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human RPB3 protein (ab181910)
    SDS-PAGE - Recombinant Human RPB3 protein (ab181910)

    15% SDS-PAGE analysis of ab181910 (3µg).

References

ab181910 has not yet been referenced specifically in any publications.

Publishing research using ab181910? Please let us know so that we can cite the reference in this datasheet.

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