Recombinant human Rsk 2 / MAPKAP Kinase 1b (mutated L608F) protein (ab172182)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% Densitometry
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: Functional Studies, WB, SDS-PAGE
Description
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Product name
Recombinant human Rsk 2 / MAPKAP Kinase 1b (mutated L608F) protein
See all Rsk 2 / MAPKAP Kinase 1b proteins and peptides -
Biological activity
Specific Activity, 140 nmol/min/mg.
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Purity
> 95 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MPLAQLADPWQKMAVESPSDSAENGQQIMDEPMGEEEINPQTEEVSIKEI AITHHVKEGHEKADPSQFELLKVLGQGSFGKVFLVKKISGSDARQLYAMK VLKKATLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFL RGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENIL LDEEGHIKLTDFGLSKESIDHEKKAYSFCGTVEYMAPEVVNRRGHTQSAD WWSFGVLMFEMLTGTLPFQGKDRKETMTMILKAKLGMPQFLSPEAQSLLR MLFKRNPANRLGAGPDGVEEIKRHSFFSTIDWNKLYRREIHPPFKPATGR PEDTFYFDPEFTAKTPKDSPGIPPSANAHQLFRGFSFVAITSDDESQAMQ TVGVHSIVQQLHRNSIQFTDGYEVKEDIGVGSYSVCKRCIHKATNMEFAV KIIDKSKRDPTEEIEILLRYGQHPNIITLKDVYDDGKYVYVVTELMKGGE LLDKILRQKFFSEREASAVLFTITKTVEYLHAQGVVHRDLKPSNILYVDE SGNPESIRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDAACD IWSLGVLLYTMLTGYTPFANGPDDTPEEILARIGSGKFSLSGGYWNSVSD TAKDLVSKMLHVDPHQRLTAALVLRHPWIVHWDQLPQYQLNRQDAPHLVK GAMAATYSALNRNQSPVLEPVGRSTLAQRRGIKKITSTAL -
Predicted molecular weight
84 kDa -
Amino acids
1 to 740 -
Tags
proprietary tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab172182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
Western blot
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.61% Tris-HCl buffer, 0.002% PMSFThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 90 kDa ribosomal protein S6 kinase 3
- CLS
- HU 3
see all -
Function
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
Tissue specificity
Expressed in many tissues, highest levels in skeletal muscle. -
Involvement in disease
Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsAutophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab172182 has not yet been referenced specifically in any publications.