Recombinant human RSK1 p90 protein (ab60880)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human RSK1 p90 protein
See all RSK1 p90 proteins and peptides -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 90 kDa ribosomal protein S6 kinase 1
- dJ590P13.1
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1
see all -
Function
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsAutophosphorylated on Ser-380, as part of the activation process. - Information by UniProt
Images
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Functional Studies - Recombinant human RSK1 p90 protein (ab60880)The specific activity of RSK1 p90 (ab60880) was determined to be 210 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human RSK1 p90 protein (ab60880)SDS PAGE analysis of ab60880 -
SDS-PAGE - Recombinant human RSK1 p90 protein (ab60880)SDS PAGE analysis of ab60880 -
Functional Studies - Recombinant human RSK1 p90 protein (ab60880)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human RSK1 p90 protein (ab60880)ab60880 on SDS-PAGE, MW ~108 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab60880 has been referenced in 1 publication.
- Kroczynska B et al. Regulatory effects of ribosomal S6 kinase 1 (RSK1) in IFN{lambda} signaling. J Biol Chem 286:1147-56 (2011). PubMed: 21075852
