Overview

Description

  • Nature

    Recombinant
  • Source

    CHO cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA
    • Molecular weight

      27 kDa
    • Amino acids

      21 to 263

Specifications

Our Abpromise guarantee covers the use of ab134626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Measured by its ability to induce Topflash reporter activity in HEK293 TCF/LEF Luc reporter cells. The ED50 for this effect is typically 0.6-1.0 μg/mL in the presence of 50 ng/ml recombinant human BMP-2.
  • Applications

    SDS-PAGE

    HPLC

  • Purity

    > 95 % SDS-PAGE.
    Purity: assessed by SDS-PAGE gel and HPLC analyses.
  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.16% Sodium phosphate, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in water to a concentration of 0.1-1.0 mg/ml.

General Info

  • Alternative names

    • CRISTIN3
    • FLJ40906
    • hRspo1
    • R spondin homolog
    • R spondin homolog (Xenopus laevis)
    • R spondin1
    • R-spondin-1
    • Roof plate specific spondin
    • Roof plate-specific spondin-1
    • RP11-566C13.1
    • RSPO
    • Rspo1
    • RSPO1_HUMAN
    see all
  • Function

    Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.
  • Tissue specificity

    Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.
  • Involvement in disease

    Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:610644]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.
  • Sequence similarities

    Belongs to the R-spondin family.
    Contains 2 FU (furin-like) repeats.
    Contains 1 TSP type-1 domain.
  • Domain

    The FU repeats are required for activation and stabilization of beta-catenin.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab134626 has not yet been referenced specifically in any publications.

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