Description

  • Product name

    Recombinant human RSPO1 protein
    See all RSPO1 proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilised Human RSPO1 at 20 µg/ml (100 µl/well) can bind Human LIMPII with a linear ranger of 31.25 - 1000 ng/ml.
  • Purity

    > 95 % SDS-PAGE.
    ab155744 was lyophilised from 0.22 µm filtered solution.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA
    • Predicted molecular weight

      28 kDa including tags
    • Amino acids

      21 to 263
    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab155744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Additional notes

    Measured by its binding ability in a functional ELISA. Immobilised Human RSPO1 at 20 µg/ml (100 µl/well) can bind Human LIMPII with a linear ranger of 31.25 - 1000 ng/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.00
    Constituents: 0.75% Glycine, 0.61% Tris
    Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • CRISTIN3
    • FLJ40906
    • hRspo1
    • R spondin homolog
    • R spondin homolog (Xenopus laevis)
    • R spondin1
    • R-spondin-1
    • Roof plate specific spondin
    • Roof plate-specific spondin-1
    • RP11-566C13.1
    • RSPO
    • Rspo1
    • RSPO1_HUMAN
    see all
  • Function

    Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.
  • Tissue specificity

    Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.
  • Involvement in disease

    Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:610644]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.
  • Sequence similarities

    Belongs to the R-spondin family.
    Contains 2 FU (furin-like) repeats.
    Contains 1 TSP type-1 domain.
  • Domain

    The FU repeats are required for activation and stabilization of beta-catenin.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • Human R-Spondin 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
  • Immobilized Human R-Spondin 1, His Tag at 5 µg/mL (100 µL/well) can bind Human SCARB2, Fc Tag with a linear range of 0.02-0.156 µg/mL.
  • SDS-PAGE analysis of reduced ab155744 stained overnight with Coomassie Blue.

References

ab155744 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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