Recombinant Human RSPO3 protein (ab192287)

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Description

  • Product name

    Recombinant Human RSPO3 protein
    See all RSPO3 proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Determined by SEC-HPLC and reducing SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    Q9BXY4

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      QNASRGRRQRRMHPNVSQGCQGGCATCSDYNGCLSCKPRLFFALERIGMK QIGVCLSSCPSGYYGTRYPDINKCTKCKADCDTCFNKNFCTKCKSGFYLH LGKCLDNCPEGLEANNHTMECVSIVHCEVSEWNPWSPCTKKGKTCGFKRG TETRVREIIQHPSAKGNLCPPTNETRKCTVDDIEGRMDEPKSCDKTHTCP PCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNW YVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKA LPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDI AVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSV MHEALHNHYTQKSLSLSPGKHHHHHH

    • Predicted molecular weight

      48 kDa including tags

    • Amino acids

      22 to 201

    • Tags

      His tag C-Terminus

    • Additional sequence information

      With a FC-6His tag at the C-terminus.

Specifications

Our Abpromise guarantee covers the use of ab192287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    HPLC

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 100% PBS

    Lyophilized from a 0.2 µM filtered solution.

General Info

  • Alternative names

    • CRISTIN1
    • hPWTSR
    • hRspo3
    • Protein with TSP type-1 repeat
    • PWTSR
    • R-spondin 3 homolog
    • R-spondin-3
    • R-spondin-3 [Precursor]
    • Roof plate specific spondin-3
    • Roof plate-specific spondin-3
    • RSPO3
    • RSPO3_HUMAN
    • Thrombospondin type I domain containing 2
    • Thrombospondin type-1 domain-containing protein 2
    • THSD2
    see all

  • Function

    Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway.

  • Tissue specificity

    Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node.

  • Sequence similarities

    Belongs to the R-spondin family.
    Contains 2 FU (furin-like) repeats.
    Contains 1 TSP type-1 domain.

  • Domain

    The FU repeats are required for activation and stabilization of beta-catenin.

  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab192287 has not yet been referenced specifically in any publications.

Publishing research using ab192287? Please let us know so that we can cite the reference in this datasheet.

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