Recombinant Human S100 alpha protein (His tag) (ab221207)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human S100 alpha protein (His tag)
    See all S100 alpha proteins and peptides

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P23297

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQK DVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

    • Predicted molecular weight

      11 kDa including tags

    • Amino acids

      1 to 94

    • Tags

      His tag C-Terminus

    • Additional sequence information

      (AAH14392).

Specifications

Our Abpromise guarantee covers the use of ab221207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.4
    Constituents: 0.61% Tris, 0.87% Sodium chloride, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

General Info

  • Alternative names

    • Bpb
    • NEF
    • Protein S100-A1
    • S-100 protein alpha chain
    • S-100 protein subunit alpha
    • S100
    • S100 alpha
    • S100 Alpha Chain
    • S100 Beta Chain
    • S100 Calcium Binding Protein A1
    • S100 Calcium Binding Protein B
    • S100 Calcium Binding Protein Beta Neural
    • S100 calcium-binding protein A1
    • S100 protein alpha polypeptide
    • S100A
    • s100a1
    • S10A1_HUMAN
    see all

  • Function

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

  • Tissue specificity

    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human S100 alpha protein (His tag) (ab221207)
    SDS-PAGE - Recombinant Human S100 alpha protein (His tag) (ab221207)

    SDS-PAGE analysis of ab221207.

    DTT-reduced Protein migrates as 11 kDa in SDS-PAGE.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab221207? Please let us know so that we can cite the reference in this datasheet.

ab221207 has not yet been referenced specifically in any publications.

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