Recombinant Human S100 alpha protein (His tag) (ab221207)

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Description

  • Product name

    Recombinant Human S100 alpha protein (His tag)
    See all S100 alpha proteins and peptides

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P23297

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQK DVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

    • Predicted molecular weight

      11 kDa including tags

    • Amino acids

      1 to 94

    • Tags

      His tag C-Terminus

    • Additional sequence information

      (AAH14392).

Specifications

Our Abpromise guarantee covers the use of ab221207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8
    Constituents: 0.61% Tris, 0.87% Sodium chloride, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • Bpb
    • NEF
    • Protein S100-A1
    • S-100 protein alpha chain
    • S-100 protein subunit alpha
    • S100
    • S100 alpha
    • S100 Alpha Chain
    • S100 Beta Chain
    • S100 Calcium Binding Protein A1
    • S100 Calcium Binding Protein B
    • S100 Calcium Binding Protein Beta Neural
    • S100 calcium-binding protein A1
    • S100 protein alpha polypeptide
    • S100A
    • s100a1
    • S10A1_HUMAN
    see all

  • Function

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

  • Tissue specificity

    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human S100 alpha protein (His tag) (ab221207)
    SDS-PAGE - Recombinant Human S100 alpha protein (His tag) (ab221207)

    SDS-PAGE analysis of ab221207.

    DTT-reduced Protein migrates as 11 kDa in SDS-PAGE.

References

ab221207 has not yet been referenced specifically in any publications.

Publishing research using ab221207? Please let us know so that we can cite the reference in this datasheet.

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