Recombinant Human SAT1 protein (ab87666)

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SDS-PAGE - Recombinant Human SAT1 protein (ab87666)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human SAT1 protein

    • Purity

      > 95 % SDS-PAGE.
      Purified by using conventional chromatography.

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MAKFVIRPAT AADCSDILRL IKELAKYEYM EEQVILTEKD LLEDGFGEHP FYHCLVAEVP KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY RGFGIGSEIL KNLSQVAMRC RCSSMHFLVA EWNEPSINFY KRRGASDLSS EEGWRLFKID KEYLLKMATE E

    Specifications

    Our Abpromise guarantee covers the use of ab87666 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • DC21
      • Diamine acetyltransferase 1
      • Diamine N acetyltransferase 1
      • EC 2.3.1.57
      • KFSD
      • KFSDX
      • Polyamine N acetyltransferase 1
      • Polyamine N-acetyltransferase 1
      • Putrescine acetyltransferase
      • SAT
      • SAT1
      • SAT1_HUMAN
      • Spermidine/spermine N(1) acetyltransferase 1
      • Spermidine/spermine N(1)-acetyltransferase 1
      • Spermidine/spermine N1 acetyltransferase 1
      • Spermidine/spermine N1 acetyltransferase alpha
      • SSAT
      • SSAT 1
      • SSAT-1
      see all

    • Function

      Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine.

    • Pathway

      Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.

    • Involvement in disease

      Keratosis follicularis spinulosa decalvans X-linked

    • Sequence similarities

      Belongs to the acetyltransferase family.
      Contains 1 N-acetyltransferase domain.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human SAT1 protein (ab87666)
      SDS-PAGE - Recombinant Human SAT1 protein (ab87666)
      ab87666 on 15% SDS-PAGE (3µg)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab87666? Please let us know so that we can cite the reference in this datasheet.

    ab87666 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    I have a question about the recombinant human SSAT protein. On the reference page, it has a good picture of Western Blot which shows the protein around 25KD. I would like to know the details of the Western Blot, such as the detailed ingredients of the gel, running buffer, loading buffer, and the exact conditions as well. I was trying to use this as a positive control for my study. But every time I only got a very very faint band with 100ng of SSAT.  Wre you able to give us any suggestion/advice or doesn’t our customer need to fill in a technical form?  Looking forward to your response.  Kind Regards    

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    Answer

    Thank you very much for contacting us with the concern of this customer. Indeed, the picture on the website is not a WEstern blot, however a SDS page where the 3ug of the protein have been loaded and than stained with coomassie blue. If the customer gets only a faint band with a coomassie blue staining, I think this is expected if only 0.1ug are loaded. It is 30 times less. If the customer however is running a Western blot, where the protein is detected with an antibody, I would expect a stronger band if the antibody is working well. Please let me know if the customer is running a Western blot, in that case I would recommend to fill the questionnaire so I can try to help to troubleshoot. If the customer is concerned that not enough protein is in the vial, I would recommend firstly to measure the concentration of the protein. This can either be done by a BSA assay, OD measurement or running a protein sample of known concentration (e.g. BSA) as a standard on the SDS Page. I hope this information is helpful. Please let me know if you have any further questions.  

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