Recombinant human SBK1 protein (ab204137)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human SBK1 protein -
Biological activity
The specific activity of ab204137 was determined to be 20 nmol /min/mg.
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Purity
> 90 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSVGCPEPEPPRSLTCCGPGTAPGPGAGVPLLTEDMQALTLRTLAASDVT KHYELVRELGKGTYGKVDLVVYKGTGTKMALKFVNKSKTKLKNFLREVSI TNSLSSSPFIIKVFDVVFETEDCYVFAQEYAPAGDLFDIIPPQVGLPEDT VKRCVQQLGLALDFMHGRQLVHRDIKPENVLLFDRECRRVKLADFGMTRR VGCRVKRVSGTIPYTAPEVCQAGRADGLAVDTGVDVWAFGVLIFCVLTGN FPWEAASGADAFFEEFVRWQRGRLPGLPSQWRRFTEPALRMFQRLLALEP ERRGPAKEVFRFLKHELTSELRRRPSHRARKPPGDRPPAAGPLRLEAPGP LKRTVLTESGSGSRPAPPAVGSVPLPVPVPVPVPVPVPVPEPGLAPQGPP GRTDGRADKSKGQVVLATAIEICV -
Predicted molecular weight
69 kDa including tags -
Amino acids
1 to 424 -
Tags
GST tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab204137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab204874 (PKI-alpha peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Sbk
- Sbk1
- SBK1_HUMAN
see all -
Function
May be involved in signal-transduction pathways related to the control of brain development. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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The specific activity of SBK1 (ab204137) was determined to be 17 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab204137
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SDS PAGE analysis of ab204137
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Sample Kinase Activity Plot showing ab204137. The specific activity of SBK1 was determined to be 20 nmol /min/mg.
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SDS-PAGE showing ab204137.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204137 has not yet been referenced specifically in any publications.