Recombinant human SCF protein (ab54340)
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Overview
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Product nameRecombinant human SCF protein
See all SCF proteins and peptides -
Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceInsect cells
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Amino Acid Sequence
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SpeciesHuman
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SequenceEGICR NRVTNNVKDV TKLVANLPKD YMITLKYVPG MDVLPSHCWI SEMVVQLSDS LTDLLDKFSN ISEGLSNYSI IDKLVNIVDD LVECVKENSS KDLKKSFKSP EPRLFTPEEF FRIFNRSIDA FKDFVVASET SDCVVSSTLS PEKDSRVSVT KPFMLPPVA
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Amino acids26 to 189
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab54340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Purity> 95 % SDS-PAGE.
Purity: 95% by SDS-PAGE and visualised by silver stain Endotoxin level: < 0.1 ng per ug of SCF -
FormLyophilised
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
None
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in 10 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can be then diluted into other aqueous buffers.
General Info
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Alternative names
- C kit ligand
- C-kit ligand
- Ckit ligand
see all -
FunctionLigand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
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Involvement in diseaseHyperpigmentation with or without hypopigmentation, familial progressive
Deafness, congenital, unilateral or asymmetric -
Sequence similaritiesBelongs to the SCF family.
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Developmental stageActs in the early stages of hematopoiesis.
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Post-translational
modificationsA soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
A soluble form exists as a cleavage product of the extracellular domain. -
Cellular localizationSecreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
- Information by UniProt
Datasheets and documents
References
ab54340 has not yet been referenced specifically in any publications.
