Recombinant human SCF protein (Active) (ab174005)
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Overview
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Product nameRecombinant human SCF protein (Active)
See all SCF proteins and peptides -
Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceHEK 293 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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SequenceEGICR NRVTNNVKDV TKLVANLPKD YMITLKYVPG MDVLPSHCWI SEMVVQLSDS LTDLLDKFSN ISEGLSNYSI IDKLVNIVDD LVECVKENSS KDLKKSFKSP EPRLFTPEEF FRIFNRSIDA FKDFVVASET SDCVVSSTLS PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS NRKAKNPPGD SSLH
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Molecular weight21 kDa
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Amino acids26 to 214
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab174005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activity
Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
The ED50 <5 ng/ml, corresponding to a specific activity of >2X105 Unit/mg. -
Applications
SDS-PAGE
Functional Studies
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Endotoxin level< 0.100 Eu/µg
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Purity> 98 % SDS-PAGE.
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FormLyophilised
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in 10 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
General Info
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Alternative names
- C kit ligand
- C-kit ligand
- Ckit ligand
see all -
FunctionLigand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
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Involvement in diseaseHyperpigmentation with or without hypopigmentation, familial progressive
Deafness, congenital, unilateral or asymmetric -
Sequence similaritiesBelongs to the SCF family.
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Developmental stageActs in the early stages of hematopoiesis.
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Post-translational
modificationsA soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
A soluble form exists as a cleavage product of the extracellular domain. -
Cellular localizationSecreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
- Information by UniProt
Images
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SDS-PAGE - Recombinant human SCF protein (Active) (ab174005)The purity of ab174005 was determined by reduced SDS-PAGE and staining overnight with Coomassie Blue. Please note: DTT-reduced Protein migrates as 30-42 kDa due to glycosylation.
Datasheets and documents
References
ab174005 has not yet been referenced specifically in any publications.
