Bioactive grade

Recombinant human SCF protein (Active) (ab174005)

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Description

  • Product name

    Recombinant human SCF protein (Active)
    See all SCF proteins and peptides

  • Biological activity

    Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
    The ED50 <5 ng/ml, corresponding to a specific activity of >2X105 Unit/mg.

  • Purity

    > 98 % SDS-PAGE.

  • Endotoxin level

    < 0.100 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P21583

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      EGICR NRVTNNVKDV TKLVANLPKD YMITLKYVPG MDVLPSHCWI SEMVVQLSDS LTDLLDKFSN ISEGLSNYSI IDKLVNIVDD LVECVKENSS KDLKKSFKSP EPRLFTPEEF FRIFNRSIDA FKDFVVASET SDCVVSSTLS PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS NRKAKNPPGD SSLH

    • Predicted molecular weight

      21 kDa

    • Amino acids

      26 to 214

Associated products

Specifications

Our Abpromise guarantee covers the use of ab174005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in 10 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • C kit ligand
    • C-kit ligand
    • Ckit ligand
    • DCUA
    • DFNA69
    • DKFZp686F2250
    • familial progressive hyperpigmentation 2
    • FPH2
    • FPHH
    • KIT ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • MGF stem cell factor
    • SCF
    • SCF_HUMAN
    • SF
    • SHEP7
    • sKITLG
    • Soluble KIT ligand
    • Steel factor
    • steel, mouse, homolog of
    • Stem cell factor
    • Stem cell factor precursor
    see all

  • Function

    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

  • Involvement in disease

    Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric

  • Sequence similarities

    Belongs to the SCF family.

  • Developmental stage

    Acts in the early stages of hematopoiesis.

  • Post-translational
    modifications

    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.

  • Cellular localization

    Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human SCF protein (Active) (ab174005)
    SDS-PAGE - Recombinant human SCF protein (Active) (ab174005)
    Human SCF, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
  • Functional Studies - Recombinant human SCF protein (Active) (ab174005)
    Functional Studies - Recombinant human SCF protein (Active) (ab174005)
    Immobilized Human SCF, Tag Free at 2 µg/mL (100 µL/well) can bind Human CD117, Fc Tag with a linear range of 0.5-4 ng/mL.
  • SDS-PAGE - Recombinant human SCF protein (Active) (ab174005)
    SDS-PAGE - Recombinant human SCF protein (Active) (ab174005)

    The purity of ab174005 was determined by reduced SDS-PAGE and staining overnight with Coomassie Blue. Please note: DTT-reduced Protein migrates as 30-42 kDa due to glycosylation.

References

ab174005 has not yet been referenced specifically in any publications.

Publishing research using ab174005? Please let us know so that we can cite the reference in this datasheet.

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