Recombinant human SDF1 protein (ab229846)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: HPLC, MS, SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human SDF1 protein
See all SDF1 proteins and peptides -
Biological activity
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Purity
> 98 % SDS-PAGE.
Expressed in E. coli then processed, refolded and purified to yield the native, secreted form of the mature chemokine. >98% pure by SDS-PAGE, HPLC, MALDI-MS and NMR analysis. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
8 kDa -
Additional sequence information
(Expressed in E.coli). Secreted form.
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Associated products
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Corresponding Antibody
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab229846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
HPLC
Mass Spectrometry
SDS-PAGE
Functional Studies
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Mass spectrometry
MALDI-TOF -
Form
Lyophilized -
Additional notes
C-X-C motif chemokine 12 alpha (CXCL12-alpha) is a 68 amino acid Cys-X-Cys chemokine that is involved in diverse cellular functions including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. CXCL12-alpha is also known as stromal cell-derived factor 1 alpha (SDF-1-alpha) and is processed from the 72 amino acid CXCL12/SDF-1 by proteolytic cleavage after secretion. The C-terminal processing to isoform CXCL12-alpha is reduced by binding to heparin and cell surface proteoglycans.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C.
Constituent: 50% Acetonitrile
Salt freeThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionAdd deionized water to desired volume, aliquot and freeze unused portion.
General Info
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Alternative names
- 12-O-tetradecanoylphorbol 13-acetate repressed protein 1
- AI174028
- C-X-C motif chemokine 12
see all -
Function
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. -
Tissue specificity
Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Developmental stage
Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney. -
Post-translational
modificationsProcessed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. -
Cellular localization
Secreted. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229846 has not yet been referenced specifically in any publications.