Recombinant Human SDHAF1 protein (ab187429)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human SDHAF1 protein -
Purity
> 85 % SDS-PAGE.
ab187429 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSRHSRL QRQVLSLYRD LLRAGRGKPG AEARVRAEFR QHAGLPRSDV LRIEYLYRRG RRQLQLLRSG HATAMGAFVR PRAPTGEPGG VGSQPDDGDS PRNPHDSTGA PETRPDGR -
Predicted molecular weight
15 kDa including tags -
Amino acids
1 to 115 -
Tags
His tag N-Terminus -
Additional sequence information
NP_001036096.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab187429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 30% Glycerol (glycerin, glycerine), 0.03% DTT, 0.002% PMSF, 69% PBS
General Info
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Alternative names
- LYR motif containing 8
- LYR motif-containing protein 8
- LYRM8
see all -
Function
Plays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II. -
Tissue specificity
Ubiquitously expressed. -
Pathway
Cofactor biosynthesis; iron-sulfur cluster biosynthesis. -
Involvement in disease
Mitochondrial complex II deficiency -
Sequence similarities
Belongs to the complex I LYR family. SDHAF1 subfamily. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab187429 has not yet been referenced specifically in any publications.