Description

  • Product name

    Recombinant human SEMA4A protein
    See all SEMA4A proteins and peptides
  • Biological activity

    Measured by its ability to bind biotinylated mouse SEMA4D in a functional ELISA.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      GGGQGPMPRVRYYAGDERRALSFFHQKGLQDFDTLLLSGDGNTLYVGARE AILALDIQDPGVPRLKNMIPWPASDRKKSECAFKKKSNETQCFNFIRVLV SYNVTHLYTCGTFAFSPACTFIELQDSYLLPISEDKVMEGKGQSPFDPAH KHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDNFLRWLHHDAS FVAAIPSTQVVYFFFEETASEFDFFERLHTSRVARVCKNDVGGEKLLQKK WTTFLKAQLLCTQPGQLPFNVIRHAVLLPADSPTAPHIYAVFTSQWQVGG TRSSAVCAFSLLDIERVFKGKYKELNKETSRWTTYRGPETNPRPGSCSVG PSSDKALTFMKDHFLMDEQVVGTPLLVKSGVEYTRLAVETAQGLDGHSHL VMYLGTTTGSLHKAVVSGDSSAHLVEEIQLFPDPEPVRNLQLAPTQGAVF VGFSGGVWRVPRANCSVYESCVDCVLARDPHCAWDPESRTCCLLSAPNLN SWKQDMERGNPEWACASGPMSRSLRPQSRPQIIKEVLAVPNSILELPCPH LSALASYYWSHGPAAVPEASSTVYNGSLLLIVQDGVGGLYQCWATENGFS YPVISYWVDSQDQTLALDPELAGIPREHVKVPLTRVSGGAALAAQQSYWP H
    • Predicted molecular weight

      72 kDa
    • Amino acids

      33 to 683
    • Tags

      His tag C-Terminus
    • Additional sequence information

      AAH20974. Extracellular domain.

Specifications

Our Abpromise guarantee covers the use of ab182683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Reconstitute for long term storage.

    pH: 7.40
    Constituents: 5% Trehalose, 95% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage

General Info

  • Alternative names

    • CORD10
    • RP11 54H19 2
    • RP35
    • SEM4A_HUMAN
    • Sema B
    • Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain 4A
    • Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4A
    • Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin)
    • Sema4a
    • SEMAB
    • Semaphorin 4A precursor
    • Semaphorin B
    • Semaphorin-4A
    • Semaphorin-B
    • SEMB
    see all
  • Function

    Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
  • Involvement in disease

    Defects in SEMA4A are the cause of retinitis pigmentosa type 35 (RP35) [MIM:610282]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in SEMA4A are the cause of cone-rod dystrophy type 10 (CORD10) [MIM:610283]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa.
  • Sequence similarities

    Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • SDS-PAGE analysis of reduced ab182683 stained overnight with Coomassie Blue.

References

ab182683 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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