Recombinant Human Semaphorin 4D/CD100 protein (ab97413)

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Key features and details

  • Expression system: CHO cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 0.100 Eu/µg
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Human Semaphorin 4D/CD100 protein
    See all Semaphorin 4D/CD100 proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Purity: Greater than 97% by SDS-PAGE gel and HPLC analyses. SDS-PAGE analysis run under non-reducing conditions shows a mixture of disulfide linked dimer and monomer.

  • Endotoxin level

    < 0.100 Eu/µg

  • Expression system

    CHO cells

  • Accession

    Q92854

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      FAPIPRITWE HREVHLVQFH EPDIYNYSAL LLSEDKDTLY IGAREAVFAV NALNISEKQH EVYWKVSEDK KAKCAEKGKS KQTECLNYIR VLQPLSATSL YVCGTNAFQP ACDHLNLTSF KFLGKNEDGK GRCPFDPAHS YTSVMVDGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIRKSPDS PDGEDDRVYF FFTEVSVEYE FVFRVLIPRI ARVCKGDQGG LRTLQKKWTS FLKARLICSR PDSGLVFNVL RDVFVLRSPG LKVPVFYALF TPQLNNVGLS AVCAYNLSTA EEVFSHGKYM QSTTVEQSHT KWVRYNGPVP KPRPGACIDS EARAANYTSS LNLPDKTLQF VKDHPLMDDS VTPIDNRPRL IKKDVNYTQI VVDRTQALDG TVYDVMFVST DRGALHKAIS LEHAVHIIEE TQLFQDFEPV QTLLLSSKKG NRFVYAGSNS GVVQAPLAFC GKHGTCEDCV LARDPYCAWS PPTATCVALH QTESPSRGLI QEMSGDASVC PDKSKGSYRQ HFFKHGGTAE LKCSQKSNLA RVFWKFQNGV LKAESPKYGL MGRKNLLIFN LSEGDSGVYQ CLSEERVKNK TVFQVVAKHV LEVKVVPKPV VAPTLSVVQT EGSRIATKVL VASTQGSSPP TPAVQATSSG AITLPPKPAP TGTSCEPKIV INTVPQLHSE KTMYLKSSDN R

    • Predicted molecular weight

      79 kDa

    • Amino acids

      24 to 734

Specifications

Our Abpromise guarantee covers the use of ab97413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    HPLC

  • Form

    Lyophilized

  • Additional notes

    This product was previously labelled as Semaphorin 4D.

    For lot specific reconstitution information please contact our Scientific Support Team.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.

    Constituent: 0.164% Sodium phosphate

  • Reconstitution
    For lot specific reconstitution information please contact our Scientific Support Team.

General Info

  • Alternative names

    • A8
    • BB 18
    • BB18
    • CD 100
    • CD100
    • CD100 antigen
    • Coll 4
    • COLL4
    • Collapsin 4
    • Collapsin4
    • GR3
    • Leukocyte activation antigen CD100
    • M sema G
    • MSEMA
    • SEM4D_HUMAN
    • Sema 4d
    • Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D
    • Sema H
    • SEMA J
    • Sema4d
    • Semacl 2
    • Semacl2
    • SemaH
    • SEMAJ
    • Semaphorin 4D
    • Semaphorin C like 2
    • Semaphorin H
    • Semaphorin J
    • Semaphorin-4D
    • Semaphorin4D
    • SemaphorinJ
    • Semcl 2
    • Semcl2
    see all

  • Function

    May play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

  • Tissue specificity

    Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.

  • Sequence similarities

    Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab97413? Please let us know so that we can cite the reference in this datasheet.

ab97413 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

  Hope this e-mail finds you well!   Kindly look into the below enquiry from one of our customer & advise us on it, enabling us to update her accordingly.   Looking forward towards your valued response on it.   With Best Regards,   My enquiry is about SEMAPHOHRIN 4D , protein, detection in Gingival crevicular fluid (GCF). GCF is present in gum/gingival tissues and is collected in microlitres and can be diluted in saline or so.   Research/pilot study regarding semaphorin 4D presence in GCF is to be done to confirm its presence in GCF so that I can buy the SEMAPHORIN 4D antibody product to further carry out my research.I want to use semaphorin 4D as a biomarker in GCF during inflammation.   Kindly send me the information as soon as possible.   Regards  

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Answer

Thank you and your customer for your inquiry. I am happy to confirm that we have 8 antibody for SEMAPHORIN 4D in our catalogue. Please find a list here: https://www.abcam.com/index.html?pageconfig=searchresults&search=SEMAPHORIN 4D&pt=1&sk=targ&sv=Semaphorin 4D&sn=Semaphorin 4D&l=0&fViewMore=1 These antibodies are all tested for human samples and a variety of applications. If your customer would like further help in choosing the right antibody for their experiment, I will be happy to help. In this case please ask your customer to provide more information concerning species and application of the planned experiments. We also offer a SEMAPHORIN 4D protein (ab97413, Recombinant fragment corresponding to amino acids 24-734 (the extracellular soluble domain) of Human Semaphorin 4D) that might be useful as positive control: Click here (or use the following: https://www.abcam.com/Recombinant-Human-Semaphorin-4DCD100-protein-ab97413.html). I hope this information was helpful. Please do not hesitate to contact me again with any further questions.

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