Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Serine/threonine-protein kinase 4/MST-1 -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204853 (IRS1 peptide) can be utilized as a substrate for assessing kinase activity
Previously labelled as Serine/threonine-protein kinase 4
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Kinase responsive to stress
- Krs2
- Mammalian STE20 like protein kinase 1
see all -
Function
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Contains 1 SARAH domain. -
Post-translational
modificationsAutophosphorylated on serine and threonine residues. -
Cellular localization
Cytoplasm. Nucleus. The caspase-cleaved form cycles between the nucleus and cytoplasm. - Information by UniProt
Images
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Functional Studies - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)The specific activity of Serine/threonine-protein kinase 4/MST-1 (ab60337) was determined to be 101.7 nmol/min/mg as per activity assay protocol and was equivalent to 953 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)SDS PAGE analysis of ab60337 -
Functional Studies - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)ab60337 on SDS-PAGE, MW ~83kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab60337 has not yet been referenced specifically in any publications.
