Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)

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Description

  • Product name

    Recombinant human Serine/threonine-protein kinase 4/MST-1

  • Expression system

    Baculovirus infected Sf9 cells

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab60337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    ab204853 (IRS1 peptide) can be utilized as a substrate for assessing kinase activity

    Previously labelled as Serine/threonine-protein kinase 4

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Kinase responsive to stress
    • Krs2
    • Mammalian STE20 like protein kinase 1
    • Mammalian STE20-like protein kinase 1
    • Mammalian sterile 20 like 1
    • MST-1
    • MST1
    • Serine/threonine kinase 4
    • Serine/threonine protein kinase Krs 2
    • Serine/threonine-protein kinase 4
    • Serine/threonine-protein kinase Krs-2
    • STE20 like kinase MST1
    • STE20-like kinase MST1
    • STK4
    • STK4_HUMAN
    • TIIAC
    • YSK3
    see all

  • Function

    Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SARAH domain.

  • Post-translational
    modifications

    Autophosphorylated on serine and threonine residues.

  • Cellular localization

    Cytoplasm. Nucleus. The caspase-cleaved form cycles between the nucleus and cytoplasm.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)
    Functional Studies - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)
    The specific activity of Serine/threonine-protein kinase 4/MST-1 (ab60337) was determined to be 101.7 nmol/min/mg as per activity assay protocol and was equivalent to 953 nmol/min/mg as per radiometric assay
  • SDS-PAGE - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)
    SDS-PAGE - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)
    SDS PAGE analysis of ab60337
  • Functional Studies - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)
    Functional Studies - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)
    SDS-PAGE - Recombinant human Serine/threonine-protein kinase 4/MST-1 (ab60337)
    ab60337 on SDS-PAGE, MW ~83kDa.

References

ab60337 has not yet been referenced specifically in any publications.

Publishing research using ab60337? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Inquiry: I would like to ask you for complete sequence of the recombinant MST1 protein. Then I would like to known if your protein is already phosphorylated on serine and threonine residues and if yes, do you know numbers of phosphorylated residues? Thanks in advance.

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Answer

Thank you for your enquiry.

I can confirm that ab60337 MST1 protein (Active) has been phosphorylated. However, we have not done any in-house characterization studies to assess the state of activation/phosphorylation on specific sites. We have performed the radiometric assay to show that the kinase is indeed active.

To manage the customers expectations, specific protein and immunogen sequences are sometimes confidential. I would appreciate if you are able to let me know why they require this sequence?

I hope this will be helpful. Please do not hesitate to contact me again if you require further information.

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Question

Hello,
I would like to ask you for complete sequence of you recombinant MST1
protein (active). I need it for interpretation of my MS analysis. Do you
also know which residues are phosphorylated in this protein? Thanks in
advance

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Answer

Thank you for your inquiry.
Please find the attached for the full sequence information for ab60337.
As far as the kinase is concerned, we do know of various sites are phosphorylated. However, we have not done any in-house characterization studies to analyse what sites specifically are phosphorylated and to what state.
This page gives an overview about all possible phosphosites:
http://www.uniprot.org/uniprot/Q13043
I hope this information is helpful and wish you good luck with your research.

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