Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, WB
Product nameRecombinant human Serine/threonine-protein kinase NIM1
The specific activity was determined to be 240 nmol/min/mg.
Purity> 90 % Densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMTAVYMNGGGLVNPHYARWDRRDSVESGCQTESSKEGEEGQPRQLTPFEK LTQDMSQDEKVVREITLGKRIGFYRIRGEIGSGNFSQVKLGIHSLTKEKV AIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLSKLHLVM EYAGGGELFGKISTEGKLSEPESKLIFSQIVSAVKHMHENQIIHRDLKAE NVFYTSNTCVKVGDFGFSTVSKKGEMLNTFCGSPPYAAPELFRDEHYIGI YVDIWALGVLLYFMVTGTMPFRAETVAKLKKSILEGTYSVPPHVSEPCHR LIRGVLQQIPTERYGIDCIMNDEWMQGVPYPTPLEPFQLDPKHLSETSTL KEEENEVKSTLEHLGITEEHIRNNQGRDARSSITGVYRIILHRVQRKKAL ESVPVMMLPDPKERDLKKGSRVYRGIRHTSKFCSIL
Predicted molecular weight74 kDa including tags
Amino acids1 to 436
Tagsproprietary tag N-Terminus
Our Abpromise guarantee covers the use of ab179497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Hypothetical protein LOC167359
- Hypothetical protein MGC42105
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab179497 has not yet been referenced specifically in any publications.