Recombinant Human Serum Albumin protein (His tag) (ab217817)

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SDS-PAGE - Recombinant Human Serum Albumin protein (His tag) (ab217817)
  • Functional Studies - Recombinant Human Serum Albumin protein (His tag) (ab217817)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human Serum Albumin protein (His tag)
    See all Human Serum Albumin proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Immobilized metal-ion affinity chromatography purification is used to purify ab217817.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P02768

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFA KTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNE CFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFY APELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKC ASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDL LECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPA DLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLA KTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGE YKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAE DYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPK EFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDD FAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

    • Predicted molecular weight

      67 kDa including tags

    • Amino acids

      25 to 609

    • Tags

      His tag C-Terminus

    • Additional sequence information

      The predicted N-terminus is Asp 25. This product is the mature full length protein from aa 25 to 609. The signal peptide and propeptide are not included (NP_000468).

  • Description

    Recombinant Human Human Serum Albumin protein (His tag)

Specifications

Our Abpromise guarantee covers the use of ab217817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 7.40
    Constituents: 90% PBS, 10% Trehalose

    Lyophilised from 0.22 µm filtered solution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

General Info

  • Alternative names

    • alb
    • ALBU_HUMAN
    • Albumin
    • Albumin (32 AA)
    • Albumin (AA 34)
    • Analbuminemia
    • Bisalbuminemia
    • Cell growth inhibiting protein 42
    • DKFZp779N1935
    • Dysalbuminemic hyperthyroxinemia
    • Growth inhibiting protein 20
    • HSA
    • Hyperthyroxinemia dysalbuminemic
    • PRO0883
    • PRO0903
    • PRO1341
    • Serum albumin
    see all

  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

  • Tissue specificity

    Plasma.

  • Involvement in disease

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.

  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.

  • Post-translational
    modifications

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Serum Albumin protein (His tag) (ab217817)
    SDS-PAGE - Recombinant Human Serum Albumin protein (His tag) (ab217817)

    SDS-PAGE analysis of DTT-reduced ab217817 followed by staining overnight with Coomassie Blue. 

    DTT-reduced protein migrates as 66 kDa in SDS-PAGE due to glycosylation.

  • Functional Studies - Recombinant Human Serum Albumin protein (His tag) (ab217817)
    Functional Studies - Recombinant Human Serum Albumin protein (His tag) (ab217817)

    Bioactivity SPR: Immobilized biotinyated Huamn FcRN Heterodimer protein on SA chip can bind ab217817, his tag with an affitinity constant of 1.78μM.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab217817? Please let us know so that we can cite the reference in this datasheet.

ab217817 has not yet been referenced specifically in any publications.

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