Recombinant Human Serum Albumin protein (His tag) (ab217817)
- Datasheet
- References
- Protocols
Overview
-
Product nameRecombinant Human Serum Albumin protein (His tag)
See all Human Serum Albumin proteins and peptides -
Protein lengthFull length protein
-
DescriptionRecombinant Human Human Serum Albumin protein (His tag)
Description
-
NatureRecombinant
-
SourceHEK 293 cells
-
Amino Acid Sequence
-
Accession
-
SpeciesHuman
-
SequenceDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFA KTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNE CFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFY APELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKC ASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDL LECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPA DLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLA KTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGE YKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAE DYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPK EFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDD FAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL
-
Molecular weight67 kDa including tags
-
Amino acids25 to 609
-
TagsHis tag C-Terminus
-
Additional sequence informationThe predicted N-terminus is Asp 25. This product is the mature full length protein from aa 25 to 609. The signal peptide and propeptide are not included (NP_000468).
-
Associated products
-
Alternative Products
- Recombinant Human Serum Albumin protein (ab201876)
- Native Human Serum Albumin protein (ab205808)
- Native Human Serum Albumin protein (FITC) (ab8030)
- Native Human Serum Albumin protein (Rhodamine) (ab8031)
- Native Human Serum Albumin protein (HRP) (ab8032)
- Native Human Serum Albumin protein (Biotin) (ab8033)
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab217817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Endotoxin level< 1.000 Eu/µg
-
Purity>95% by SDS-PAGE .
-
FormLyophilised
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 90% PBS, 10% Trehalose
Lyophilised from 0.22 µm filtered solution. -
ReconstitutionReconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
General Info
-
Alternative names
- alb
- ALBU_HUMAN
- Albumin
see all -
FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
-
Tissue specificityPlasma.
-
Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
-
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localizationSecreted.
- Information by UniProt
Images
-
SDS-PAGE - Recombinant Human Serum Albumin protein (His tag) (ab217817)SDS-PAGE analysis of DTT-reduced ab217817 followed by staining overnight with Coomassie Blue.
DTT-reduced protein migrates as 66 kDa in SDS-PAGE due to glycosylation.
Datasheets and documents
References
ab217817 has not yet been referenced specifically in any publications.
