Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, WB
Product nameRecombinant human Serum Amyloid A (mutated D60N + H71R) protein
See all Serum Amyloid A proteins and peptides
Tested by its ability to down- regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4 μM. Schreiber, BM. et al. Biochem J. 1999 Nov 15; 344 Pt 1:7-13
Purity>= 98 % SDS-PAGE.
>=98% by SDS-PAGE and HPLC analyses.
Endotoxin level< 0.100 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS NARENIQRFF GRGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY
Predicted molecular weight12 kDa
Modificationsmutated D60N + H71R
Our Abpromise guarantee covers the use of ab50232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 µg/ml. ab50232 can be used as a WB positive control in conjunction with ab687.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionPlease reconstitute this product in water to a concentration of 1mg/ml.
- Amyloid fibrils
- SAA 2
- Serum amyloid A1 isoform 2
RelevanceFunction: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
Anti-Serum Amyloid A antibody  (ab687) at 0.5 µg/ml +
Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (ab50232) at 0.01 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab50232 has not yet been referenced specifically in any publications.