Bioactive grade

Recombinant human Serum Amyloid A protein (ab50232)

Description

  • Product name

    Recombinant human Serum Amyloid A protein
    See all Serum Amyloid A proteins and peptides
  • Biological activity

    Tested by its ability to down- regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4 μM. Schreiber, BM. et al. Biochem J. 1999 Nov 15; 344 Pt 1:7-13

  • Purity

    >= 98 % SDS-PAGE.
    >=98% by SDS-PAGE and HPLC analyses.
  • Endotoxin level

    < 0.100 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS NARENIQRFF GRGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY
    • Predicted molecular weight

      12 kDa
    • Modifications

      unmodified N60 + R71

Specifications

Our Abpromise guarantee covers the use of ab50232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Form

    Lyophilised
  • Additional notes

    For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 µg/ml. ab50232 can be used as a WB positive control in conjunction with ab687.
     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Please reconstitute this product in water to a concentration of 1mg/ml.

General Info

  • Alternative names

    • Amyloid fibrils
    • SAA 2
    • Serum amyloid A1 isoform 2
    • TP53I4
    • Amyloid fibril protein AA
    • Amyloid protein A
    • Fibrils
    • MGC111216
    • OC
    • OTTMUSP0000004557
    • PIG 4
    • PIG4
    • SAA
    • SAA 1
    • SAA_HUMAN
    • SAA1
    • SAA2
    • serum amyloid A
    • Serum amyloid A 1
    • Serum amyloid A protein precursor
    • Serum amyloid A1 isoform 1
    • Serum amyloid protein A(4-101)
    • Tumor protein p53 inducible protein 4
    see all
  • Relevance

    Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.

Images

  • Anti-Serum Amyloid A antibody [115] (ab687) at 0.5 µg/ml + Recombinant human Serum Amyloid A protein (ab50232) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 1 minute

References

ab50232 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your patience in this matter. I have verified that ab50232 (Serum Amyloid A protein (Active)) does not contain sodium azide or any other added preservatives. Please do not hesitate to contact us again if you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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