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Immunology Adaptive Immunity T Cells Cytotoxic Cells
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Recombinant Human SET/TAF-I protein (ab167879)

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Western blot - Recombinant Human SET/TAF-I protein (ab167879)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human SET/TAF-I protein
    • Purity

      > 90 % SDS-PAGE.
      ab167879 is purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      Q01105
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD
      • Predicted molecular weight

        36 kDa including tags
      • Amino acids

        1 to 290
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)
      • Anti-SET/TAF-I antibody (ab92872)
      • Anti-SET/TAF-I antibody (ab97596)

    Specifications

    Our Abpromise guarantee covers the use of ab167879 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Additional notes

      This product was previously labelled as SET.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • 2PP2A
      • HLA DR associated protein II
      • HLA-DR-associated protein II
      • I 2PP2A
      • I-2PP2A
      • I2PP2A
      • IGAAD
      • Inhibitor of granzyme A activated DNase
      • Inhibitor of granzyme A-activated DNase
      • Inhibitor of GzmA-activated DNase
      • inhibitor-2 of protein phosphatase-2A
      • IPP2A2
      • PHAPII
      • Phosphatase 2A inhibitor I2PP2A
      • protein phosphatase type 2A inhibitor
      • Protein SET
      • Set
      • SET nuclear oncogene
      • SET translocation
      • SET translocation (myeloid leukemia-associated)
      • SET_HUMAN
      • TAF I
      • TAF IBETA
      • TAF-I
      • TAFI
      • Template activating factor I
      • Template-activating factor I
      • Template-Activating Factor-I, chromatin remodelling factor
      see all
    • Function

      Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
    • Tissue specificity

      Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
    • Involvement in disease

      Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
    • Sequence similarities

      Belongs to the nucleosome assembly protein (NAP) family.
    • Domain

      The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.
    • Post-translational
      modifications

      Isoform 2 is phosphorylated on Ser-15 and Thr-23.
      Isoform 2 is acetylated on Lys-11.
      Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
      N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.
    • Cellular localization

      Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.
    • Target information above from: UniProt accession Q01105 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Recombinant Human SET/TAF-I protein (ab167879)
      Western blot - Recombinant Human SET/TAF-I protein (ab167879)
      15% SDS-PAGE analysis of ab167879 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab167879? Please let us know so that we can cite the reference in this datasheet.

    ab167879 has not yet been referenced specifically in any publications.

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