Description

  • Product name

    Recombinant human SFRP1 protein
  • Biological activity

    Biological Activity :Determined by its ability to inhibit BMP-2 proliferation effect of ATDC-5 cells. The expected ED50 is 0.3-0.5 µg/ml.
  • Purity

    > 95 % SDS-PAGE.
    Purity: Greater than 97% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Endotoxin level

    < 0.100 Eu/µg
  • Expression system

    HeLa cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      SEYDYVSFQS DIGPYQSGRF YTKPPQCVDI PADLRLCHNV GYKKMVLPNL LEHETMAEVK QQASSWVPLL NKNCHAGTQV FLCSLFAPVC LDRPIYPCRW LCEAVRDSCE PVMQFFGFYW PEMLKCDKFP EGDVCIAMTP PNATEASKPQ GTTVCPPCDN ELKSEAIIEH LCASEFALRM KIKEVKKENG DKKIVPKKKK PLKLGPIKKK DLKKLVLYLK NGADCPCHQL DNLSHHFLIM GRKVKSQYLL TAIHKWDKKN KEFKNFMKKM KNHECPTFQS VFK
    • Amino acids

      32 to 314

Associated products

Specifications

Our Abpromise guarantee covers the use of ab64445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    This protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with 200µl of sterile water.

General Info

  • Alternative names

    • Frizzled related protein 1
    • FRP
    • FRP 1
    • FRP-1
    • FRP1
    • FrzA
    • SARP 2
    • SARP-2
    • SARP2
    • Secreted apoptosis related protein 2
    • Secreted apoptosis-related protein 2
    • Secreted frizzled related protein
    • Secreted frizzled related protein 1
    • Secreted frizzled-related protein 1
    • SFRP 1
    • sFRP-1
    • SFRP1
    • SFRP1_HUMAN
    see all
  • Function

    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
  • Tissue specificity

    Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
  • Sequence similarities

    Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • Domain

    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization

    Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
  • Information by UniProt

References

This product has been referenced in:

  • Marcos S  et al. Secreted frizzled related proteins modulate pathfinding and fasciculation of mouse retina ganglion cell axons by direct and indirect mechanisms. J Neurosci 35:4729-40 (2015). Read more (PubMed: 25788689) »
  • Valcz G  et al. Myofibroblast-derived SFRP1 as potential inhibitor of colorectal carcinoma field effect. PLoS One 9:e106143 (2014). Read more (PubMed: 25405986) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

The lyophilized protein can be stored at -20°C to -80°C
It is stable for up to 3 months at 4oC

Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.

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Answer

Thank you for your enquiry. I can confirm that the ab64445 SFRP1 protein is a glycosylated protein with a total of 283 amino acids and the glycosylation is located at residue 173. This particular product needs to be glycosylated in order for it to be active, which we have demonstrated by the activity tests. This product is also derived from HeLa cells as opposed to E.coli, so will be glycosylated. I am sorry we do not test for further details of the glycosylation, however, according to literature. it states the following: 'Analysis of glycosylation sites showed that sFRP-1 contains a relatively large carbohydrate moiety on Asn(173) (approximately 2.8 kDa).' I hope this will be helpful to you. If you have any additional questions, please do not hesitate to let me know.

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