Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      PGDYKDDDDK PAGVRGAPCE AVRIPMCRHM PWNITRMPNH LHHSTQENAI LAIEQYEELV DVNCSAVLRF FLCAMYAPICTLEFLHDPIK PCKSVCQRAR DDCEPLMKMY NHSWPESLAC DELPVYDRGV CISPEAIVTD LPEDVKWIDI TPDMMVQERP LDVDCKRLSP DRCKCKKVKP TLATYLSKNY SYVIHAKIKA VQRSGCNEVT TVVDVKEIFK SSSPIPRTQV PLITNSSCQC PHILPHQDVL IMCYEWRSRM MLLENCLVEK WRDQLSKRSI QWEERLQEQR RTVQDKKKTA GRTSRSNPPK PKGKPPAPKP ASPKKNIKTR SAQKRTNPKR V
    • Molecular weight
      39 kDa including tags
    • Amino acids
      19 to 346
    • Tags
      DDDDK tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

    Mass Spectrometry

  • Endotoxin level
    < 1.000 Eu/µg
  • Mass spectrometry
    LC-MS/MS
  • Purity
    > 95 % Densitometry.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    pH: 7.40
    Constituents: 99% Phosphate Buffer, 0.43% Sodium chloride

  • Reconstitution
    Add 200µl of deionized water to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage.

    Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

General Info

  • Alternative names
    • Frizzled protein
    • Frizzled protein human endometrium
    • FRP 4
    • FRP4
    • FrpHE
    • human endometrium
    • PYL
    • Secreted frizzled-related protein 4
    • sFRP-4
    • Sfrp4
    • SFRP4_HUMAN
    see all
  • Function
    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
  • Tissue specificity
    Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).
  • Sequence similarities
    Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • Domain
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization
    Secreted. Cytoplasmic in ovarian tumor cells.
  • Information by UniProt

Images

  • 12% SDS-PAGE analysis of ab167942

    Lane 1: reduced and boiled sample, 2.5μg/lane
    Lane 2: non-reduced and non-boiled sample, 2.5μg/lane

References

ab167942 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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