Description

  • Product name

    Recombinant human SGK1 protein
    See all SGK1 proteins and peptides
  • Biological activity

    Specific Activity: 117 nmol/min/mg.

  • Purity

    > 95 % SDS-PAGE.
    Purity: >95% as determined by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      73 kDa
    • Amino acids

      60 to 431
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      Recombinant fragment, corresponding to amino acids 60-end of Human SGK1.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab204849 (AKT1 peptide) can be utilized as a substrate for assessing kinase activity

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • OTTHUMP00000017247
    • Serine/threonine protein kinase SGK
    • Serine/threonine protein kinase Sgk1
    • Serine/threonine-protein kinase Sgk1
    • Serum and glucocorticoid regulated kinase
    • Serum/glucocorticoid regulated kinase
    • Serum/glucocorticoid regulated kinase 1
    • Serum/glucocorticoid-regulated kinase 1
    • SGK
    • SGK 1
    • SGK1
    • Sgk1 variant i3
    • SGK1_HUMAN
    see all
  • Function

    Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.
  • Tissue specificity

    Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Domain

    Isoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids.
  • Post-translational
    modifications

    Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
    Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid.
  • Cellular localization

    Cell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation.
  • Information by UniProt

Images

  • The specific activity of SGK1 (ab60883) was determined to be 99 nmol/min/mg as per activity assay protocol
  • SDS PAGE analysis of ab60883
  • SDS PAGE analysis of ab60883
  • Sample Kinase Activity Plot.
  • ab60883 on SDS-PAGE, MW ~73 kDa.

References

ab60883 has not yet been referenced specifically in any publications.

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